摘要
目的观察慢性复合应激后大鼠海马神经元FGF-2表达的变化,探讨慢性复合应激对FGF-2表达的影响及其与海马学习记忆功能的联系。方法将成年雄性Wistar大鼠随机分为复合应激组和正常对照组。采用垂直旋转、睡眠剥夺、捆绑和夜间光照四种应激源每天交替无规律刺激复合应激组动物达6周,制作慢性复合应激动物模型。然后利用Morris水迷宫测试大鼠空间学习记忆能力,并运用免疫组织化学方法观察海马区神经元FGF-2表达的变化。结果慢性复合应激后,应激组动物在Morris水迷宫内寻找隐蔽平台所需时间(即潜伏期)较对照组缩短(P<0.05);应激组大鼠海马FGF-2免疫反应阳性神经元的表达增多(P<0.05)。结论慢性复合应激可增强大鼠空间学习记忆能力,而FGF-2在海马神经元的表达增加可能是其影响因素之一。
Objective To explore the effect of chronic muhiple-stress on learning and memory and the expression of FGF-2 in hippocampal neurons of rats. Methods Adult male Wistar rats were randomly divided into two groups: the multiple stressed group and the control group. Rats in the multiple stressed group were ir- regularly and alternatively exposed to the situation of vertical revolution, sleep deprivation, restraint, and night illumination for 6 weeks to prepare a chronic multiple-stressed model. Then learning and memory performance of rats was measured by Morris water maze and the expression of FGF-2 in hippocampal neurons of rats was de- tected by immunohistochemical method. Results After chronic multiple-stress, compared with the control group, the escape latency to the hidden platform in Morris water maze was shortened in stressed group ( P 〈 0. 05 ). The expression of FGF-2 in hippocampal neurons of the multiple stressed rats was significantly higher than that of the control group ( P 〈 0.05 ). Conclusion The increased expression of FGF-2 in hippocampus after chronic multiple-stress may be participated in the enhancing effect of chronic multiple-stress on learning and memory.
出处
《咸宁学院学报(医学版)》
2010年第1期5-8,共4页
Journal of Xianning Univarsity(medical Sciences)