中空纤维酶膜反应器制取异麦芽低聚糖的新工艺

New Process of Producing Isomalto- Oligosaccharides with Hollow Fiber Membrane Reactor

采用聚砜中空纤维酶膜反应器连续化工艺流程，以转葡萄糖苷酶和真菌淀粉酶双酶协同作用于麦芽低聚糖转苷制取异麦芽低聚糖。通过对酶膜反应系统稳态操作，确定转苷酶膜反应停留时间，双酶补加量范围及反应温度，试验结果表明：酶膜反应器连续化生产工艺，大大缩短反应周期，提高生产能力，又节省酶用量，同时显著改善了产品的质量，分枝性低聚糖含量达６４％

A polysalfone hollow fiber membrane reactor (HFMR) was used to produce continuously isomaltooligosaccharides.The maltooligosaccarides were transformed into isomaltooligosaccharides by biozyme and transglucosidase.Through the orthogonal experiments of the continuous production by HFMR,the dosages of two enzymes,the reaction temperature and the reaction time were optimized.The results showed that comparing to the conventional system,HFMR system significantly saved the enzyme dosage,greatly shortened the reaction time and increased the productivity. Moreover,the quality of the products was much improved,and the yield of branching-saccharides went up to 64%.