旱柳体外培养与直接分化再生系统的建立

Culture in Vitro and Establishment of Regeneration System of Direct Differentiation of Salix matsudana Koidz

给出了旱柳体外培养的方法：9月份至12月份采集1～2a生枝条上带腋芽的茎段，经过筛选确定MS＋0．5mg／L6-BA＋0．1mg／LNAA为最佳诱导培养基，平均每个芽点产生的不定芽数量为3．5个，20d后苗高为1．8cm；不定芽在MS＋0．1mg／L6-BA＋0．2mg／LNAA培养基中继代增殖及生长较好，为适宜的增殖培养基；在形成的不定芽中有些不定芽起源于单株苗的切口基部，因此，初步建立了旱柳的直接分化再生系统，可为旱柳的遗传转化等研究奠定基础．经过壮苗处理的幼苗在MS＋0．2mg／LNAA培养基上的生根率达100％，且生根量多，幼苗生长健壮．生根苗移栽至V（草炭土）：V（河沙）为3：1的混合基质中，60d后成活率为90％左右．

A method is described for culture in vitro of Salix matsudana. On Murashige and Skoog＇ s medium （MS） containing benzylaminopurine（BA） at 0.5 mg/L and naphthaleneacetic acid（NAA） at 0.1 mg/L 3.5-fold shoot multiplication from stem with one axillary bud collected on 1 -2 a branch can be achieved, and the adventitious buds were 1.8 cm in length after 20 d. On MS containing BA at 0.1 mg/L and NAA at 0.2 mg/L was the best subculture and proliferation medium, adventitious buds produced and some of them directly originated from the basal of the single shoot were in possessed of the well growth and proliferation, therefore, regeneration system of direct differentiation of Salix matsudana Koidz was primarily established, which can lay a foundation on genetic transformation research of Salix matsudana Koidz. The produced shoots in this way were rooted on MS containing only NAA（0.2 mg/L）, the rooting percentage was 100%. When the plantlets were transplanted to proportion of 3 ： 1 （peat soil ： river sand） of mixed matrix,the survival rate could reach 90% after 60 days.