新疆紫草低极性部位GC-MS/MS化学成分分析

GC-MS/MS analysis of the low polarity components from the root of Arnebia euchroma

目的研究新疆紫草低极性部位的主要化学成分。方法新疆紫草用乙醇提取,提取液回收乙醇后用石油醚萃取,石油醚萃取部位分为乙酰化处理和未进行处理两部分,采用GC-MS/MS法进行测定,与标准谱库进行比较分析,并用峰面积归一化法计算各成分的质量百分数。结果共检出14种化学成分,其中含棕榈酸(9.31%)和硬脂酸(5.03%),并含有γ-棕榈酸内酯(2.56%)和十四酸(2.02%)等。结论新疆紫草低极性部位主要成分为长链脂肪酸及其内酯。长链脂肪酸为首次从新疆紫草中分离得到;脂肪酸内酯为首次从紫草科植物中分离得到。

Objective To investigate the low polarity components from the root of Arnebia euchroma. Methods The EtOH extracts of the root of Arnebia euchroma was extracted with petroleum ether. Part of the petroleum ether extraction was acetylated. The components were separated and identified from both parts by GC-MS/MS and elucidated by the comparison with the standard mass spectral data. The relative contents in percentage were calculated using the area normalization method. Results 14 compounds were identi- fied, including hexadecanoic acid （ 9.31% ） and octadeeanoie acid （5.03 % ）, along withγ-hexadecanuic acid lactone （2.56%） and tetradecanoic acid（2.0：2% ） etc. Conclusion The main components in the low polarity components of the root of Arnebia euchroma are fatty acid and fatty acid lactone. Fatty acid have been isolated from the root of Arnebia euchroma for the first time, fatty acid lactone have been firstly isolated from Boraginaceae plants.