肼苯哒嗪对大肠癌Lovo细胞及RUNX-3基因的影响

Effect of Hydralazine on the Proliferation of Colorectal Cancer Lovo Cells and Expression of RUNX-3 Gene

目的:观察肼苯哒嗪对大肠癌Lovo细胞增殖作用的影响,探讨肼苯哒嗪对大肠癌Lovo细胞中抑癌基因RUNX-3的转录调节作用及其对细胞生长和凋亡的影响。方法:应用不同浓度(0,10,20,40,80,160μmol/L)的肼苯哒嗪,于不同作用时间(24,48,72,96h)处理人大肠癌Lovo细胞,采用四甲基偶氮唑蓝(MTT)法测定肼苯哒嗪作用下Lovo细胞的生存率,倒置显微镜观察细胞的形态学变化;采用RT-PCR法检测肼苯哒嗪对RUNX-3 mRNA表达的影响。结果:①不同药物浓度组在同一作用时间下,细胞生存率差异有统计学意义(P<0.05);同一药物浓度不同作用时间组之间(24-96h)的细胞生存率无统计学意义(P>0.05);②RUNX-3 mRNA在大肠癌Lovo细胞中的表达随浓度的增加而增强。结论:肼苯哒嗪有抑制人大肠癌Lovo细胞生长的作用,抑制作用呈浓度依赖性;可以促进RUNX-3 mRNA的表达,从而促进大肠癌Lovo细胞的凋亡。

Objective：To investigate the effect of hydralazine on the growth of human colorectal cancer Lovo cells and the transcription of the RUNX-3 gene in Lovo cells. Methods： Human colorectal cancer Lovo cells were treated by hydralazine at different concentrations （0, 10, 20, 40, 80, and 160 μmol/L） respectively at different time （24, 48, 72, and 96 hours respectively）. The growth rate of the cells was detected by MTT assay. The expression of RUNX-3 mRNA was analyzed by RT-PCR. Results： ①The proliferation of Lovo cells was inhibited by hydralazine at concentrations of 10-160 μmol/L in a concentration-dependent manner in stead of time-dependent manner （P0.05）. ②The expression of RUNX-3 mRNA was up-regulated as the concentration increased. Conclusion： Hydralazine can inhibit the proliferation of Lovo cells and promote the expression of RUNX-3 mRNA in a concentration-dependent manner （10-160 μmol/L）.