hCGβ-TT-IRES-CD40L真核表达载体的构建

Construction of Beta-hCG-TT-IRES-CD40L Eukaryotic Expression Vector

目的:构建含有分子佐剂CD40L的真核表达质粒hCGβ-TT,以提高hCGβ的免疫原性。方法:用分段PCR的方法构建了hCGβ-TT,然后插入双元真核表达质粒IRES的A位点,将CD40L插入pIRES的B位点构建hCGβ-TT-IRES-CD40L。结果:经测序所克隆的基因hCGβ-TT和CD40L序列正确。结论:利用分子佐剂CD40L与hCGβ-TT的连接,构建了hCGβ-TT-IRES-CD40L真核细胞表达质粒,为检测hCGβDNA免疫避孕疫苗的免疫原性及抗生育作用奠定了基础。

Objective：To construct hCGβ-TT eukaryotic expression plasmid containing CD40L molecular adjuvant to enhance the immunogenicity of hCGβ. Methods： The eukaryotic expression plasmid of hCGβ-TT was constructed and inserted into the dual IRES eukaryotic expression plasmid in locus A, and CD40L was inserted into locus B to generate hCGβ-TT-IRES-CD40L eukaryotic expression plasmid. Results： hCGβ-TT and CD40L were identified by cloning and sequencing of hCGβ-TT-IRES-CD40L plasmid. Conclusion： The hCGβ-TT-IRES-CD40L eukaryotic expression plasmid was established successfully for the further study of contraceptive vaccine hCGβ DNA.