丁苯酞对阿尔茨海默病模型大鼠海马GFAP和VEGF的影响

Effect of butylphthalide on the expression of GFAP and VEGF in the hippocampus of rats with Alzheimer's disease

目的:研究阿尔茨海默病(AD)模型大鼠海马中胶质纤维酸性蛋白(GFAP)、血管内皮生长因子(VEGF)的表达及丁苯酞对其的影响。方法:成年雄性SD大鼠60只,随机分为模型组、丁苯酞组及对照组,每组20只,通过海马内注射β-淀粉样蛋白1-42建立阿尔茨海默病大鼠模型,喂养60d后取各组大鼠脑组织,进行GFAP和VEGF免疫组织化学染色。结果:与对照组比较,模型组大鼠海马各区GFAP阳性细胞明显增多,VEGF表达减少,差异有统计学意义(P<0.01);与模型组比较,丁苯酞组大鼠海马各区GFAP阳性细胞数明显减少,VEGF表达增加,差异有统计学意义(P<0.05)。结论:丁苯酞可能通过减少海马GFAP表达,增加VEGF的表达,从而达到保护AD模型大鼠神经血管单元的作用。

Objective To determine the expression of glial fibrillary acidic protein （GFAP） and vascular endothelial growth factor （ VEGF ） in the hippocampus of rats with Alzheimer＇ s disease （ AD ）, and to determine the effect of butylphthalide on them and its significance. Methods Sixty male adult rats were randomly divided into a model group, a Butylphthalide group, and a control group. AD models were established by injecting β-amyloid protein 1-42 into the hippocampus of rats. Sixty days later,the rats were sacrificed and both sides of the hippocampus were sectioned for immunohistochemistry. Results Positive cells of GFAP in the hippocampus of the model group increased and the expression of VEGF decreased statistically, compared with the control group （ P 〈 0.01 ）. The positive cells of GFAP in the hippocampus of the butylphthalide group decreased and the expression of VEGF increased significantly, compared with the model group （ P 〈 0.05 ）. Conclusion Butylphthalide may protect the neuron-vascular unit of the hippocampus of Alzheimer model rats by inhibiting the expression of GFAP and increasing the expression of VEGF.