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高糖诱导NRK-52E细胞甲状旁腺素相关肽及其受体1表达及氯沙坦干预研究 被引量:2

Effect of losartan on expression of PTHrP and PTH1R induced by high glucose in NRK-52E cells
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摘要 为探讨甲状旁腺素相关肽(PTHrP)及其受体1(PTH1R)在高糖诱导大鼠。肾小管导管上皮细胞株NRK-52E表达及氯沙坦对其表达干预作用。应用RT—PCR及Western印迹检测空白组(0mmol/L),正常葡萄糖组(5mmol/L)、中高浓度葡萄糖组(16.7mmol/L)及高浓度葡萄糖组(25mmol/L)中PTHrP及PTH1R在NRK-52E细胞表达。然后10μmoL/L氯沙坦及25mmol/L葡萄糖分别干预72h,应用Western印迹检测PTHrP及PTH1R表达情况。结果发现高浓度葡萄糖能上调PTHrP/PTHlR蛋白(PTHrP:0.63±0.12,0.68±0.06,1.02±0.11和1.04±0.08;PTH1R:0.88±O.05,0.87±0.10,1.05±0.11和1.12±0.09)及mRNA表达(PTHrP:0.66±0.08,0.84±0.13,1.57±0.15和1.73±0.21;PTH1R:0.26±0.08,0.28±0.07,2.35±0.10和2.47±0.05)。氯沙坦能显著下调高浓度葡萄糖状态下PTHrP及PTH1R表达(PTHrP0.74±0.15,PTH1R0.98±0.06,均P〈0.01)。提示氯沙坦可以抑制高浓度葡萄糖诱导PTHrP/PTH1R的表达水平,从而可能影响肾小管导管上皮转分化。 To study the effect of losartan on high glucose up-regulated expression of parathyroid hormone- related peptide (PTHrP)and its type 1 receptor (PTH1 R)in NRK-52E cells. The expression of PTHrP and PTH1R were detected by RT-PCR and Western blot in control group (0 mmol/L glucose) , normal glucose group (5 mmol/ L glucose), moderately high glucose group ( 16.7 mmol/L glucose ) , high glucose group ( 25 mmol/L glucose), and after intervention by 10 μmol/L losartan for 72 h (only Western blot). The expression of PTHrP and PTH1R were up-regulated by high glucose (PTHrP mRNA : 0. 66 ± 0. 08 , 0. 84 ±0. 13,1.57±0. 15, and 1.73±0.21 ; PTHrP protein :0.63±0.12,0.68±0.06,1.02±0. 11, and 1.04±0.08 ; PTH1R mRNA :0.26±0.08,0.28±0.07,2.35± 0.10,and 2.47±0.05; PTH1R protein:0.88±0.05,0.87±0. 10, 1.05±0. 11 ,and 1. 12±0.09) ,and losartan inhibited the effects of high glucose (PTHrP 0.74±0.15 ,PTH1R 0.98±0.06,both P〈0.01 ). The results suggest that losartan could inhibit the expression of PTHrP and PTH1R induced by high glucose in NRK-52E.
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2010年第2期111-113,共3页 Chinese Journal of Endocrinology and Metabolism
基金 基金项目:广西科技厅自然科学基金资助项目(桂科自0991294) 广西北海市科学研究与技术开发计划项目(北科合字200901059)
关键词 甲状旁腺素相关肽 甲状旁腺素受体1 氯沙坦 NRK-52E细胞 葡萄糖 PIHrP PTH1R Losartan NRK-52E cells High glucose
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参考文献7

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同被引文献22

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