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高糖诱导NRK-52E细胞甲状旁腺素相关肽及其受体1表达及氯沙坦干预研究 被引量：2

Effect of losartan on expression of PTHrP and PTH1R induced by high glucose in NRK-52E cells
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摘要 为探讨甲状旁腺素相关肽（PTHrP）及其受体1（PTH1R）在高糖诱导大鼠。肾小管导管上皮细胞株NRK-52E表达及氯沙坦对其表达干预作用。应用RT—PCR及Western印迹检测空白组（0mmol／L），正常葡萄糖组（5mmol／L）、中高浓度葡萄糖组（16．7mmol／L）及高浓度葡萄糖组（25mmol／L）中PTHrP及PTH1R在NRK-52E细胞表达。然后10μmoL／L氯沙坦及25mmol／L葡萄糖分别干预72h，应用Western印迹检测PTHrP及PTH1R表达情况。结果发现高浓度葡萄糖能上调PTHrP／PTHlR蛋白（PTHrP：0．63±0．12，0．68±0．06，1．02±0．11和1．04±0．08；PTH1R：0．88±O．05，0．87±0．10，1．05±0．11和1．12±0．09）及mRNA表达（PTHrP：0．66±0．08，0．84±0．13，1．57±0．15和1．73±0．21；PTH1R：0．26±0．08，0．28±0．07，2．35±0．10和2．47±0．05）。氯沙坦能显著下调高浓度葡萄糖状态下PTHrP及PTH1R表达（PTHrP0．74±0．15，PTH1R0．98±0．06，均P〈0．01）。提示氯沙坦可以抑制高浓度葡萄糖诱导PTHrP／PTH1R的表达水平，从而可能影响肾小管导管上皮转分化。 To study the effect of losartan on high glucose up-regulated expression of parathyroid hormone- related peptide （PTHrP）and its type 1 receptor （PTH1 R）in NRK-52E cells. The expression of PTHrP and PTH1R were detected by RT-PCR and Western blot in control group （0 mmol/L glucose） , normal glucose group （5 mmol/ L glucose）, moderately high glucose group （ 16.7 mmol/L glucose ） , high glucose group （ 25 mmol/L glucose）, and after intervention by 10 μmol/L losartan for 72 h （only Western blot）. The expression of PTHrP and PTH1R were up-regulated by high glucose （PTHrP mRNA ： 0. 66 ± 0. 08 , 0. 84 ±0. 13,1.57±0. 15, and 1.73±0.21 ; PTHrP protein ：0.63±0.12,0.68±0.06,1.02±0. 11, and 1.04±0.08 ; PTH1R mRNA ：0.26±0.08,0.28±0.07,2.35± 0.10,and 2.47±0.05; PTH1R protein：0.88±0.05,0.87±0. 10, 1.05±0. 11 ,and 1. 12±0.09） ,and losartan inhibited the effects of high glucose （PTHrP 0.74±0.15 ,PTH1R 0.98±0.06,both P〈0.01 ）. The results suggest that losartan could inhibit the expression of PTHrP and PTH1R induced by high glucose in NRK-52E.
作者 梁华晟 钟宇华
机构地区 广西医科大学第九附属医院内分泌科
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2010年第2期111-113,共3页 Chinese Journal of Endocrinology and Metabolism
基金 基金项目：广西科技厅自然科学基金资助项目（桂科自0991294） 广西北海市科学研究与技术开发计划项目（北科合字200901059）
关键词 甲状旁腺素相关肽 甲状旁腺素受体1 氯沙坦 NRK-52E细胞 葡萄糖 PIHrP PTH1R Losartan NRK-52E cells High glucose
分类号 R587.2 [医药卫生—内分泌]
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参考文献7

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同被引文献22

  • 1Hastings RH, Montgrain PR, Quintana R, et al. Cell cycle actions of parathyroid hormone-related protein in non-small cell lung carcinoma. Am J Physiol Lung Cell Mol Physiol, 2009, 297: L578-L585.
  • 2Fujinaka Y, Sipula D, Garcia-Ocana A, et al. Characterization of mice doubly transgenic for parathyroid hormone-related protein and murine placental lactogen : a novel role for placental lactogen in pancreatic beta- cell survival. Diabetes, 2004,53 : 3120-3130.
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中华内分泌代谢杂志
2010年 第2期

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