TNFα对小鼠肾上腺皮质细胞系Y1增殖、凋亡的影响

Effects of TNFα on proliferation and apoptosis of mouse adrenal cortex cell line Y1

目的:探讨肿瘤坏死因子α(TNFα)对小鼠肾上腺皮质细胞系Y1增殖、凋亡的影响。方法:不同浓度、不同作用时间的TNFα处理小鼠肾上腺皮质细胞系Y1;MTT法检测细胞的增殖;台盼蓝拒染法检测细胞增殖抑制;流式细胞仪检测细胞的凋亡水平;分光光度法检测细胞caspase8/3的变化。结果:不同浓度的TNFα处理Y1细胞不同时间后,细胞的增殖能力明显下降(P<0.05);细胞凋亡水平明显提高(P<0.05);细胞caspase8/3水平明显升高(P<0.05)。结论:TNFα可抑制Y1细胞增殖,并通过提高caspase8/3的活性而诱导及促进细胞凋亡。

Objective：To investigate the effects of tumor necrosis factor α（TNFα） on proliferation and apoptosis of mouse adrenal cortex cell lines Y1.Methods：The mouse adrenal cortex cell line Y1 was treated with TNFα at different concentrations during different duration.The cells proliferation was tested by MTT assay and cytostasis was detectd by Trypan Blue Coloring Method.Flow cytometry was used to observe the cells apoptosis level.The changes of Caspase8/3 of the cells were detected by spectrophotometry.Results：After being treated with TNFα of different concentration during different duration,reproductive activities of Y1 cells were significantly inhibited（P0.05）;the apoptosis and Caspase8/3 levels of Y1 cells were both remarkably highered（P0.05）.Conclusion：TNFα can inhibit proliferation of Y1 cells,and induce and promote the apoptosis by increasing caspase8/3 activity.