摘要
考察不同剂量的H2O2作用于小鼠成纤维细胞(NIH3T3)后,细胞脂质过氧化、存活率、DNA片段化的变化情况,并研究谷胱甘肽过氧化物酶(GPX)的模拟物6A,6B-环己胺-6A′,6B′-硒桥联-β-CD(6-CySeCD)抗H2O2诱导小鼠成纤维细胞损伤的能力.结果表明:H2O2对NIH3T3细胞有严重损伤;6-CySeCD能有效保护细胞,防止紫外线引起的损伤.
The antioxidant effect of 6A,6A'-cyclohexylamine-6B,6B'-selenium-bridged-β-cyclodextrin (6-CySeCD), a new mimic of glutathione peroxidase (GPX) , was confirmed with a model system in which mouse fibroblast cell NIH3T3 were damaged by H2PO2. The cell damage was characterized in terms of the lipid peroxidation of the cells, cell viability, DNA lysis rate. The injury effects of H2 O2 and protection effects of 6-CySeCD on the cells were studied via the model system. The results show the 6-CySeCD could be able to protect cells from the damage induced by H2O2.
出处
《吉林大学学报(理学版)》
CAS
CSCD
北大核心
2010年第2期329-332,共4页
Journal of Jilin University:Science Edition
基金
国家自然科学基金(批准号:20572035
30970608)
吉林大学基本科研业务费项目(批准号:200903093)