HPLC同时测定消栓通络胶囊中阿魏酸、芦丁和丹酚酸B的含量

Determination of ferulic acid,rutin and salvianolic acid B in Xiaoshuan Tongluo Capsule by HPLC

目的:建立同时测定消栓通络胶囊(川芎,丹参,黄芪,三七,槐花,等)中阿魏酸、芦丁和丹酚酸B含量的高效液相色谱方法。方法:以Linksil C18(250mm×4.6mm,5μm)色谱柱为分析柱,甲醇-乙腈-0.1%冰醋酸溶液(25∶5∶70)为流动相,检测波长为320nm,流速:1.0mL/min。结果:3种成分分离良好,阿魏酸、芦丁和丹酚酸B的线性范围分别为0.004～0.08μg、0.432～8.64μg、0.088～1.76μg,相关系数均为0.9999。平均加样回收率(n=5)分别为98.9%、100.1%、98.2%;RSD分别为1.1%、1.5%、1.4%。结论:本方法简便、快速,结果准确可靠,适用于消栓通络胶囊的质量控制。

AIM：To develop a method for determining the contents of ferulic acid,rutin and salvianolic acid B in Xiaoshuan Tongluo Capsule（Rhizoma Chuanxiong,Radix et Rhizoma Salviale miltiorrhizale,Radix Astragali,Radix et Rhizoma notoginseng,Flos Sophorale,etc.） METHODS：The column was Linksil C18（250mm×4.6 mm,5 μm）.The mobil phase was methanol-acetonitrile -0.1% acetic acid（25：5 ：70）.The detection wave-lenght was set at 320 nm.The flow rate was 1.0 mL/min.RESULTS：The calibration curves of ferulic acid,rutin and salvianolic acid B were in good linearity over the ranges of 0.004-0.08 μg,0.432-8.64 μg,0.088-1.76 μg,respectively（r=0.999 9）.And average recoveries（n=5） were 98.9 %,100.1 % and 98.2 %,respectively and RSD were 1.1 %,1.5 %,1.4 %.CONCLUSION：The method is simple,rapid,reliable and can be used for the quality control of Xiaoshuan Tongluo Capsule.