HPLC测定千金子和千金子霜中两种泻下成分的含量

Determination of purgative constituents in Semen Euphorbiae and Semen Euphorbiae Pulveratun by HPLC

目的:对不同地区千金子药材及其炮制品千金子霜中泻下成分续随二萜酯和千金二萜醇二乙酸苯甲酸酯的含量进行测定。方法:采用高效液相色谱法,流动相为甲醇-水(68∶32),kromasil C18柱(150mm×4.6mm,5μm),柱温:40℃,检测波长:280nm。结果:续随二萜酯和千金二萜醇二乙酸苯甲酸酯分别在0.200μg～1.200μg、0.206μg～1.651μg范围内,线性关系良好,r=0.9997和r=0.9999。不同产地千金子中所含两种成分的量略有差别,续随二萜酯的质量分数在0.4508%～0.6251%,多数在0.4500%以上。千金二萜醇二乙酸苯甲酸酯的质量分数在0.6923%～0.7529%,多数在0.7200%以上。千金子霜中两种成分的质量分数分别在0.1568%～0.2211%(多数在0.2000%以下)和0.2322%～0.2830%(多数在0.3000%以下)。结论:建立了高效液相色谱法测定千金子脂肪油中两种泻下成分含量的方法,该方法准确、可靠,可用于毒性中药千金子及其炮制品的质量控制。

AIM：To determine the contents of euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae and Semen Euphorbiae Pulveratun from different habitats.METHODS：A kromasil C18 column（150 mm×4.6 mm,5 μm） was used with methanol-water（68 ：32） as the mobile phase and UV detection was at 280 nm.RESULTS：The linear ranges of euphorbiasteroid and lathyrol diadetate benzozte were from 0.200 μg to 1.200 μg and 0.206 μg to 1.651 μg（r=0.999 7,r=0.9999）.The contents of euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae from different habitats ranged from 0.450 8% to 0.625 1% and 0.692 3% to 0.752 9%.The contents of two kinds of compositions in Semen Euphorbiae Pulveratun ranged from 0.156 8% to 0.221 1% and 0.232 2% to 0.283 0%.CONCLUSION：The method for quantitating euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae is accurate and reliable,and can be used to control the quality of toxicity Semen Euphorbiae and Semen Euphorbiae Pulveratun.