正常乳腺组织和浸润性乳腺癌组织中淋巴增强因子-1(LEF-1)转录因子的蛋白质表达水平的分析

Analysis of the differential of protein expression level of LEF-1 transcription factor between normal breast tissue and invasive breast cancer

目的通过实验对正常乳腺组织和浸润性乳腺癌组织中淋巴增强因子-1(LEF-1)转录因子的蛋白质表达水平进行分析,观察其差异。方法对从手术获得的新鲜乳腺组织做冰冻切片,经H&E染色分析鉴定出正常乳腺组织和乳腺浸润癌组织。用显微切割方法获取目标组织,再采用蛋白质免疫印迹法(Westen blot)检测正常乳腺组织和乳腺浸润癌组织中LEF-1转录因子的蛋白质表达差异。结果乳腺浸润癌组织中的LEF-1转录因子的蛋白质表达水平高于正常乳腺组织中的LEF-1转录因子的蛋白质表达水平。结论LEF-1转录因子的表达与乳腺癌的浸润相关,正常乳腺组织和乳腺浸润癌组织中的LEF-1转录因子的蛋白质表达水平的分析是研究LEF-1转录因子的调控作用机理的基础。

Objective This study analyse the level of protein expression of LEF- 1 transcription factor in normal breast tissue and invasive breast cancer tissue. Methods Fresh pathology material from surgical operation were performed frozeen dissecting, and then stain by H＆E staining method. Examining the slice and divided tussues into two groups-normal breast tissue and invasive breast can- cer. To examine the difference of protein expression level of LEF-1 transcription factor in the normal mammary gland tissue and inva- sive breast cancer tissue by Western blot method. Results The level of protein expression of LEF- 1 transcription factor in the invasive breast cancer tissue was higher than LEF-1 transcription factor in the normal mammary gland tissue. Condusion Expression of LEF- 1 transcription factor are interrelated with infiltration of breast cancer. This research is a basis for further study of the regulatory mech- anism of LEF-1 transcription factor in the invasive breast cancer.