阿霉素诱导大鼠扩张型心肌病模型的建立

Adriamycin-induced dilated cardiomyopathy model of rat

目的建立稳定可靠的扩张型心肌病(dilated cardiomyopathy,DCM)动物模型,以供基础实验研究。方法选用雄性Wistar大鼠,分为正常对照组和DCM模型组,DCM组接受腹腔注射生理盐水溶解的阿霉素2.5 mg/(kg.week),稀释浓度1 mg/ml;正常组腹腔注射等量生理盐水。每周根据体重调整阿霉素剂量,用药6周后,停药观察2周。记录大鼠体重的变化及死亡率,检查超声心动图检查、血浆脑利钠肽(BNP)水平、测量左心室内径、观察心肌病理切片、电镜、Masson染色结果。结果DCM模型组体重增长缓慢,低于正常对照组,出现死亡。心脏超声心动图示模型组左室舒张期末径(left ventricular end diastolic diameter,LVEDD)、左室收缩期末径(left ventricular end systolic diameter,LVESD)和左室射血分数(left ventricular ejection fraction,LVEF)、左室短轴缩短率(fraction shortening,FS)。各项指标明显低于正常对照组,P<0.01。模型组大鼠血浆BNP水平高于正常对照组,P<0.01。模型组左心室内径大于正常对照组P<0.01。心肌病理切片、电镜观察、Masson染色结果符合DCM改变。结论该制造扩张型心肌病动物模型的方式方法成功率高,造模周期较短,结果可靠,可以做为基础研究的动物模型。

Objective To establish a stable and reliable animal models with dilated cardiomyopathy （dilated cardiomyopathy, DCM） for basic experiments.Methods Male Wistar rats were divided into normal control group and DCM model group,DCM group received intraperitoneal injection of saline soluble doxorubicin 2.5 mg/（kg. week）, dilute the concentration of 1 mg/ml; normal group, intraperitoneal injection, tales doses volume of normal saline. Weekly according to body weight adjusted dose of doxorubicin, medication 6 weeks after the withdrawal observed 2 weeks. Records of body weight changes and mortality, Check echocardiography, plasma brain natriuretic peptide （BNP） levels, left ventricular diameter, myocardial pathology, electron microscopy. Results DCM model group, the slow weight gain, lower than the control group,. Model of cardiac echocardiography shows left ventricular end dias- tolic diameter（ LVEDD）, left ventrieular end systolic diameter, （LVESD） and left ventricular ejection fraction （LVEF）, left ventricular fractional shortening （b-S）. The index was significantly lower than the control group, P 〈 0.01. Model group, plasma BNP levels higher than the normal control group, P 〈 0.01. Model group, left ventricular internal diameter greater than the normal control group, P 〈 0.01. Myocardial pathology, electron microscopy, results in line with DCM to change. Conclusion The way of make animal model of dilated cardiomyopathy has high success rate, short-cycle modeling and reliable results. It can serve as animal models for basic research.