高糖对细胞膜流动性的影响

The effects of high level of glucose on membrane fluidity

目的探讨高糖环境对ECV 304细胞膜流动性的影响。方法体外培养ECV 304细胞,利用葡萄糖建立高糖环境。将细胞随机分为正常细胞组、高糖组、甘露醇组。甘露醇组为高渗对照组。高糖组加入葡萄糖,终浓度为35mmol.L-1,甘露醇组加入甘露醇终浓度为10 mmol.L-1,正常组细胞不加处理因素。加入处理因素24 h后,收集细胞。利用电子自旋共振波谱仪测定细胞膜流动性和细胞SOD活力、MDA含量、抗超氧阴离子自由基能力、抑制羟基自由基能力。结果与正常组比较,高糖组SOD活性、抗超氧阴离子自由基能力、抑制羟基自由基能力均显著低于正常组和甘露醇组,而MDA含量则高于正常组和甘露醇组(p<0.05);高糖组细胞膜流动性显著低于正常组和甘露醇组;正常组细胞膜流动性高于甘露醇组。结论高糖会造成由氧化应激介导的ECV 304细胞损伤,高糖环境可以降低细胞膜流动性。

Objective To investigate the impact of high glucose environment on the ECV 304 cell membrane fluidity. Methods The ECV 304 cells were cultured in vitro, and high glucose environment were built up by making use of glucose. The cells were randomly divided into normal cells group, high glucose group and nmnnitol group. Clucose was added into the high glucose group, the final concentration was 35M. Mannitol was added into the mannitol group, and the final concentration was 25M, no treatment factor was ad- ministered to the normal cells group. 24h after administering the treatment factors, all cells were collected. The cell membrane fluidity of ECV 304 cells were measured by electron spin resonance and the superoxide dismutase （SOD） activities, the ability of anti-superoxide anion, the suppression ability of hydroxyl radical and the level of malondialdehyde （MDA） was measured by kits. Results Com- pared to the normal cells group and the mannitol group, The activities of SOD, the ability of anti-superoxide anion, and the suppression ability of hydroxyl radical was significantly decreased in high glucose group, while the level of MDA, production of lipid peroxidation, was significantly increased （ P 〈 0.05） ; membrane fluidity was significantly lower than the normal group and the mannitol group; membrane fluidity of the normal cells group is higher than mannitol group. Conclusion High glucose environment can cause damage to ECV 304 cells, which is mediated by oxidative stress;high glucose environment can reduce the cell membrane fluidity.