重组N蛋白抗原检测PPRV抗体ELISA的研究——试剂盒阴阳性临界值的测定及其与OIE参考实验室试剂盒的比较

Studies on ELISA with a Recombinant N-protein as Antigen for the Detection of PPRV Antibody

对临床上采集的244份不同背景的羊血清样本,用纯化的重组N蛋白为包被抗原建立的检测小反刍兽疫病毒(PPRV)抗体的间接ELISA进行检测,运用统计学方法摸清了检测结果的分布规律,并同时用OIE参考实验室抗体检测试剂盒进行检测,结果表明,两种检测方法的符合率为91.73%。利用TG-ROC软件分析了ELISA抗体检测临界值,该试剂盒与国外试剂盒相比,其相对特异性和敏感性分别为98.6%和85.4%。

A rapid inexpensive enzyme-linked immunosorbent assay（ELISA） for quantitation of antibodies to peste des petits ruminants virus （PPRV） in serum was developed using a recombinant PPRV nucleprotein.244 serum samples from goat were detected for the antibody to PPRV by using the developed ELISA and the OIE PPR Laboratory＇s ELISA antibody test kit was used simultaneously for comparison. The developed ELISA was correlat- ed well （91.73%）with the OIE ELISA. Performance and optimal cutoff values for the ELISA were determined by using the Two Graph-ROC function of the CMDT software package, and it was found that the relative specificity and sensitivity of the developed ELISA were 98.6% and 85.4% respectively compared with the OIE Reference Laboratory＇s ELISA kit.