猪繁殖与呼吸综合征、猪2型圆环病毒病二联基因疫苗的构建

Construction of Associated DNA Vaccine of PRRS and PCV2 Disease

将猪繁殖与呼吸综合征病毒(PRRSV)长春分离株的ORF5基因克隆至真核表达载体pIRES的CMV的启动子下游,并用猪二型圆环病毒的内蒙古分离株的ORF2基因替代pIRES的neo基因,构建真核表达质粒。通过West-ernblot以及IFA检测方法鉴定,所构建的重组质粒能在BHK细胞内进行有效的转录,并表达了目的蛋白。

In this study, ORF5 gene of the changchun PRRSV strain was cloned into pIRES expressing vector , ORF2 gene of the Mongolia PCV2 strain substitute neo gene of pIRES expressing vector.The recombinant DNA vaccine was transcribed and expressed in BHK cells effectively through Western blot and IFA.