海金沙挥发油的指纹图谱和GC/MS分析

Analysis on fingerprint and GC/MS of volatile oil from Lygodium japonicum

通过水蒸气蒸馏法制备海金沙挥发油,用GC/MS分析了挥发油乙醚萃取物的化学成分,构建了海金沙的气相色谱指纹图谱,并进行了方法学考察。指纹图谱由14个特征峰构成,不同产地样品的图谱有一定差异;指纹图谱相对保留时间的精密度、稳定性和重现性的RSD分别在1.8%,1.76%和2.45%以下。挥发油用GC/MS共检出20个化学成分,主要成分是α-油酸单甘油酯和油酸二羟基乙酯,相对含量分别为47.82%和42.77%。

The volatile oil from Lygodium japonicum was prepared by water vapor distillation method and its chemistry composition was analysed by GC/MS. The fingerprint of the volatile oil was established by gas chromatography and its methodology was investigated. The fingerprint is consist of fourteen characteris- tic peaks and fingerprint of sample from different producing area was different. RSD of the precision, stability and repeatability of fingerprint＇s relative retention times are 1.8%, 1.76% and 2.45% ,respective- ly. Twenty chemical substances in volatile oil was analysed by GC-MS, main constituent is α-monoolein and oleic acid 2-hydroxyethyl ester,and relative content is 47.82% and 42.77% ,respectively.