原发性胆汁性肝硬化患者抗核抗体特殊荧光模型分析及诊断价值

The clinical significance of the anti-nuclear antibody special fluorescence patterns in primary biliary cirrhosis

目的探讨原发性胆汁性肝硬化(PBC)患者抗核抗体(ANA)特殊荧光模型特点及其诊断价值。方法对62例PBC患者血清及515例对照血清用欧蒙公司试剂检测ANA特殊荧光模型及其滴度并用免疫印迹法确认。结果62例PBC患者中49例有ANA的特殊荧光模型,阳性率为79.0%,与对照组比较有统计学意义(P<0.05),而且83.7%的特殊荧光模型都为≥1 000的高滴度。特殊荧光模型混合模型的靶抗体检出率要高于单一特殊荧光模型。结论ANA的特殊荧光模型与PBC密切相关,尤其是其中的混合模型(≥2种的特殊荧光模型)对于PBC具有重要诊断价值。

Objective To further investigate the characteristic and the diagnostic value of the anti-nuclear antibody special fluores-cence patterns in primary biliary cirrhosis. Methods The anti-nuclear antibody special fluoresence patterns in serum samples of 62 primary biliary cirrhosis （PBC） and 515 control cases were tested by indirect immune fluorescence and the positive result were detected respectively by immune blotting assay. Results 49 of the 62 cases in primary biliary cirrhosis were positive for anti-nuclear antibody special fluorescence patterns, the sensibility is 79% , which was significantly higher than that in ,other control（ P 〈 0.05 ）, and their specifieity for PBC was 94.2%, moreover 83.7% showed high titer （ ≥1： 1000） . The target antigen in anti-nuclear antibody special mix fluorescence patterns was higher than that in special single fluorescence patterns, and the specjficity in, mix pattems was near to 100%; Conclusions The characteristic of the anti-nuclear antibody special fluorescene pattems has a close relation with primary biliiary cirrosis, especially the mix patterns of it has an important diagnostic value for PBC.