麦芽糖浓度和渗透压对游动放线菌生长及阿卡波糖生物合成的综合影响

Comprehensive Effects of Acarbose Concentration and Medium Osmotic Pressure on Acarbose Production in Actinoplanes sp. Fermentation

阿卡波糖(1)是游动放线菌(Actinoplanes sp.)的次级代谢产物,其生成与菌体生长具有相关性。发酵液渗透压对细胞生长及1的生物合成影响显著。本研究考察了渗透压对1生物合成的影响,结果表明通过补料将发酵前期渗透压维持在300mOsm/kg,中后期维持400～500mOsm/kg,麦芽糖浓度维持6%,1生物合成水平大幅提高,50L发酵罐效价为3360mg/L,提高了50%以上。

Acarbose biosynthesis showed a growth-associated type of product formation in Actinoplanes sp. fermentation. It was found that medium osmotic pressure affected both cell growth and acarbose biosynthesis notably. A significant increase of acarbose production was achieved when osmotic pressure was kept at 300 mOsm/kg in the basal medium and at 400 - 500 mOsm/kg during the fermentation process as well as the maltose concentration was kept about 6%. The titer of acarbose reached to 3 360 mg/L in a 50 L fermentor, which was increased by 50%.