人血浆中拉米夫定、齐多夫定、奈韦拉平的LC-MS／MS法测定

Determination of Lamivudine,Zidovudine and Nevirapine in Human Plasma by LC-MS/MS

建立了LC-MS/MS法同时测定人血浆中拉米夫定、齐多夫定、奈韦拉平。以克来夫定为内标,血浆样品用甲醇沉淀蛋白处理,采用ESI源正离子模式、多反应监测进行定量分析。三组分的线性范围均为20～5000ng/ml,方法回收率为96.45%～101.5%,批内、批间RSD分别小于6.4%和6.7%。已用于健康志愿者同时服用3种药物的药动学研究。

A LC-MS/MS method was established for the simultaneous determination of lamivudine, zidovudine and nevirapine in human plasma with clevudine as the internal standard. The samples were treated by protein precipitation with methanol. A tandem mass spectrometric detection was conducted using multiple reaction monitoring （MRM） under positive ionization mode with an electrospray ionization （ESI） interface. The calibration curves of lamivudine, zidovudine and nevirapine were linear in the concentration range of 20 - 5 000 ng/ml. The method recovery was between 96.45 % - 101.5 %. The RSDs of intra- and inter-batch were less than 6.4 % and 6.7 %. The method has been successfully applied to the pharmacokinetics study in healthy volunteers.