摘要
探讨消炎痛对人结肠癌细胞株的抗肿瘤效应,以阐明其抗肿瘤机理。方法:分别用消炎痛(终浓度100~800μm)或消炎痛+5-氟脲嘧啶(5-Fu)处理体外培养的人结肠癌细胞株RKO,采用DNA凝胶电泳,MTT法,流式细胞仪分析技术,检测消炎痛对细胞增殖和凋亡的影响。结果:消炎痛可显著抑制细胞增殖和诱导细胞凋亡,并呈剂量———时间依赖性;消炎痛可协同5-Fu抗肿瘤细胞增殖效应。结论:消炎痛抑制细胞增殖和诱导细胞凋亡是其抗结肠癌作用机制的重要方面之一。
Objective:To explore the antitumor effect of indomethacin on human colon adenocarcinoma cells and determine the influence of indomethacin on cancer cells proliferation and apoptosis. To try to elucidate antitumor mechanism of Indomethacin. Methods:Human RKO cell line were cultured seperatery in vitro, Indomethacin was administered alone (final concentration 100~800μm) or altogether with 5-Fu(50μm).The proliferation and apoptosis of cells were monitored. Results:Indomethacia can inhibit significantry the proliferation of RKO cells and induce apoptosis with a“dose-time”-dependent manner.A synergic effect of inhibiting cancer cells proliferation was observed when combined with Indomethacia and 5-Fu.These results indicated that Indomethacia can suppress human colon adenocarcinoma proliferation and induce apoptosis.
出处
《中国现代医学杂志》
CAS
CSCD
1998年第12期1-3,共3页
China Journal of Modern Medicine
基金
湖南省自然科学基金
关键词
消炎痛
结肠癌
细胞增殖
细胞凋亡
Indomethacia
Colon carcinoma
Cell proliferation
Apoptosis