消炎痛抑制人结肠癌细胞增殖和诱导凋亡的研究

STUDY OF INDOMETHACIN INHIBIT THE PROLIFERATION AND INDUCE APOPTOSIS OF HUMAN COLON ADENOCARCINOMA CELL LINE RKO

探讨消炎痛对人结肠癌细胞株的抗肿瘤效应，以阐明其抗肿瘤机理。方法：分别用消炎痛（终浓度１００～８００μｍ）或消炎痛＋５－氟脲嘧啶（５－Ｆｕ）处理体外培养的人结肠癌细胞株ＲＫＯ，采用ＤＮＡ凝胶电泳，ＭＴＴ法，流式细胞仪分析技术，检测消炎痛对细胞增殖和凋亡的影响。结果：消炎痛可显著抑制细胞增殖和诱导细胞凋亡，并呈剂量———时间依赖性；消炎痛可协同５－Ｆｕ抗肿瘤细胞增殖效应。结论：消炎痛抑制细胞增殖和诱导细胞凋亡是其抗结肠癌作用机制的重要方面之一。

Objective:To explore the antitumor effect of indomethacin on human colon adenocarcinoma cells and determine the influence of indomethacin on cancer cells proliferation and apoptosis. To try to elucidate antitumor mechanism of Indomethacin. Methods:Human RKO cell line were cultured seperatery in vitro, Indomethacin was administered alone (final concentration 100～800μm) or altogether with 5-Fu(50μm).The proliferation and apoptosis of cells were monitored. Results:Indomethacia can inhibit significantry the proliferation of RKO cells and induce apoptosis with a“dose-time”-dependent manner.A synergic effect of inhibiting cancer cells proliferation was observed when combined with Indomethacia and 5-Fu.These results indicated that Indomethacia can suppress human colon adenocarcinoma proliferation and induce apoptosis.