血管紧张素Ⅱ诱导肾小球系膜细胞纤维连接蛋白产生的实验研究

EFFECTS OF ANGIOTENSIN Ⅱ ON FIBRONECTIN BIOSYNTHESIS IN HUMAN MESANGIAL CELLS

目的：观察不同浓度的血管紧张素Ⅱ（ＡｎｇⅡ）刺激后系膜细胞ＡｎｇⅡ受体基因表达和分泌纤维连接蛋白（ＦＮ）的情况。方法：从６月龄人胎肾培养系膜细胞，经ＡｎｇⅡ刺激后用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）方法检测胎肾系膜细胞ＡＴ１和ＡＴ２受体ｍＲＮＡ的表达情况，分别用ＥＬＩＳＡ和ＲＴ－ＰＣＲ方法检测系膜细胞产生的ＦＮ和基因表达。结果：①经１０－８、１０－７、１０－６和１０－５ｍｏｌ／Ｌ的ＡｎｇⅡ刺激４８ｈ后，系膜细胞的ＡＴ１受体ｍＲＮＡ表达均有升高，并呈一定的浓度依赖性；而ＡＴ２受体ｍＲＮＡ仅在１０－５ｍｏｌ／Ｌ浓度的ＡｎｇⅡ刺激下才有微弱表达。②１０－７、１０－６和１０－５ｍｏｌ／Ｌ的ＡｎｇⅡ能明显促进系膜细胞产生ＦＮ和ＦＮ的ｍＲＮＡ表达的上调，并与ＡｎｇⅡ刺激浓度呈正相关。结论：ＡｎｇⅡ刺激后可使系膜细胞的ＡＴ１受体基因表达和ＦＮ的产生明显增加，并呈一定的浓度依赖性。

OBJECTIVE To investigate the effects of angiotensin Ⅱ (Ang Ⅱ)on fibronectin(FN)biosynthesis in human glomerular mesangial cells. METHODOLOGY Cultured human glomerular mesangial cells from 6 month fetus were treated with Ang Ⅱ at different concentrations(10 -8 ,10 -7 ,10 -6 and 10 -5 mol/L respectively)for 48 hours.The mRNA expressions of Ang Ⅱ receptor type 1(AT1)and type 2(AT2)genes in glomerular mesangial cells were examined by RT PCR.FN expression in glomerular mesangial cells was detected by both ELISA and RT PCR. RESULTS The mRNA expression of AT1 receptor gene was markedly enhanced by Ang Ⅱ in mesangial cells in a dose dependent manner,while AT2 receptor was not.FN biosynthesis was increased by Ang Ⅱ in mesangial cells in a dose dependent pattern. CONCLUSION It is suggested that Ang Ⅱ,via its AT1 receptor,stimulates FN secretion of human mesangial cells in a dose dependent pattern.