“血瘀证”下急性心肌梗死模型与单纯急性心肌梗死模型大鼠心脏血流动力学及心电图变化的比较

Comparison of hemodynamics and electrocardiogram between acute myocardial infarction models with blood stasis and simple acute myocardial infarction models in rats

目的:观察"血瘀证"下急性心肌梗死模型与单纯急性心肌梗死模型大鼠心脏血流动力学及心电图的变化,阐明单纯急性心肌梗死模型与"血瘀证"下急性心肌梗死模型之间的差异。方法:Wistar大鼠随机分为空白对照组、急性血瘀模型组、急性心肌梗死假手术组、单纯急性心肌梗死模型组及"血瘀证"下急性心肌梗死模型组(在大鼠急性"血瘀"情况下复制急性心肌梗死模型)(每组12只),测定各组大鼠的左室收缩压(LVSP)、左室舒张末压(LVEDP)、左室压力变化最大速率(±dp/dtmax)、颈总动脉收缩压(SBP)、舒张压(DBP)及平均动脉压(MAP)等各项血流动力学指标。同时测定大鼠心率(HR)及心电图(ECG)V5导联和Ⅱ导联ST段等参数,并与单纯急性心肌梗死模型进行对比。结果:①大鼠"血瘀证"下急性心肌梗死模型与单纯急性心肌梗死模型在SBP、DBP、MAP、LVSP、LVEDP及±dp/dtmax等指标上差异无显著性(P>0.05);②大鼠"血瘀证"下急性心肌梗死模型的HR加快及V5、Ⅱ导联心电图ST段抬高程度均明显大于单纯急性心肌梗死模型组(P<0.05或P<0.01)。结论:大鼠"血瘀证"下急性心肌梗死模型与单纯急性心肌梗死模型在心电图变化上存在明显差异,在血流动力学各项指标上无明显差异,提示采用"血瘀证"下急性心肌梗死模型来评价治疗胸痹心痛中药的药效学更为合理。

Objective To study the changes of hemodynamics and electrocardiogram of the acute myocardial infarction models with blood stasis and the simple acute myocardial infarction models in rats and elucidate the difference between the acute myocardial infarction models with blood stasis and the simple acute myocardial infarction models in rats.Methods Wistar rats were randomly divided into control group,acute blood stasis models group,acute myocardial infarction and sham operation group,acute myocardial infarction models group and acute myocardial infarction models with blood stasis group（n=12）.The acute myocardial infarction models under the status of the acute blood stasis in rats were built to determine the parameters of the left ventricular systolic pressure（LVSP）,left ventricular end-diastolic pressure（LVEDP）,maximum left ventricular rise rate and maximum left ventricular fall rate（±dp/dt max）,and the pressure of carotid artery including systolic blood pressure（SBP）,diastolic blood pressure（DBP） and mean artery pressure（MAP）.In addition,the parameters of electrocardiogram（ECG） including heart rate（HR） and ST segment with V5 lead and Ⅱlead were also measured.Results ① When the acute myocardial infarction models with blood stasis in rats and the simple acute myocardial infarction models were comparatively studied,the results showed that there were no obvious differences between them in SBP,DBP,MAP,LVSP,LVEDP and ±dp/dt max（P〉0.05）;② The increasing extents of the HR and ST segment with V5 lead and Ⅱlead of the acute myocardial infarction models with blood stasis in rats were much higher than those of the simple acute myocardial infarction models（P〈0.05 or P〈0.01）.Conclusion The obvious differences are shown on the parameters of ECG,but there are no obvious differences in the hemodynamics when the acute myocardial infarction models with blood stasis and the simple acute myocardial infarction models are comparatively studied.The results show that it is much reasonable to evaluate pharmacodynamics of traditional Chinese drug which treat thoracic obstruction cardialgia in the acute myocardial infarction models with blood stasis in rats.