TREM-1、TNF-α和IL-8在单核细胞源性泡沫细胞中的表达

Expressions of TREM-1,TNF-α and IL-8 in foam cells derived from macrophages

目的:探讨髓系细胞触发受体1(TREM-1)、肿瘤坏死因子α(TNF-α)和白细胞介素8(IL-8)在巨噬细胞源性泡沫细胞产生过程中的表达情况。方法:人单核细胞株(U937)经佛波酯(PMA)诱导贴壁后,分为PMA诱导组、低密度脂蛋白(LDL)诱导组及氧化型低密度脂蛋白(ox-LDL)诱导组。应用RT-PCR法检测上述3组细胞中TREM-1mRNA表达;用ELISA法检测上述3组细胞培养上清中TNF-α和IL-8含量。结果:与PMA诱导组、LDL组细胞比较,ox-LDL组细胞TREM-1mRNA表达水平均明显增高(P<0.05);ox-LDL组培养上清中TNF-α、IL-8含量明显高于LDL组及PMA诱导组(P<0.05)。结论:TREM-1、TNF-α和IL-8在动脉粥样硬化(AS)发生发展中可能存在相互诱导、相互协同作用,共同参与AS的形成。

Objective To detect the expressions of triggering receptor expressed on myeloid cells-1（TREM-1）,TNF-α and IL-8 of foam cells derived from macrophages.Methods Human U937 cell strain was induced by PMA and then was divided into PMA group,LDL group and ox-LDL group.RT-PCR was applied to measure the mRNA expression of TREM-1 in each group and ELISA was used to detect the contents of TNF-α and IL-8 in culture supernatant in each group.Results Compared with PMA group and LDL group,the TREM-1 mRNA expression in ox-LDL group was significantly increased（P〈0.05）;the TNF-α and IL-8 contents in culture supernatant in ox-LDL group were also significantly increased（P〈0.05）.Conclusion TNF-α,IL-8,and TREM-1 maybe play an important synergetic effect in the process of atherosclerosis.