人子宫颈癌组织中MMP-26、MMP-9和TIMP-4的表达及其意义

Expressions of MMP-26,MMP-9 and TIMP-4 in cervical carcinoma tissues and their significances

目的:检测人子宫颈癌组织中基质金属蛋白酶-26(MMP-26)、基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶组织抑制因子-4(TIMP-4)的表达,探讨其与临床病理特征的关系。方法:应用免疫组织化学SP法检测10例正常子宫颈组织、6例宫颈原位癌和79例宫颈浸润癌组织中MMP-26、MMP-9及TIMP-4的表达。结果:MMP-26在宫颈原位癌和浸润性癌中阳性表达率分别为83.33%和82.28%,较正常子宫颈组织中表达明显增强(P<0.05);MMP-26表达与子宫颈癌的肌层浸润深度和淋巴结转移有密切关联(P<0.05),随着子宫颈癌浸润深度的增加,MMP-26阳性表达增强;淋巴结转移组MMP-26蛋白阳性表达率明显高于无淋巴结转移组(P<0.05)。MMP-9在宫颈浸润癌组织中阳性率为68.35%,MMP-9表达与子宫颈癌病理分级有密切关联,其在Ⅲ级子宫颈癌组织中的表达明显高于Ⅰ和Ⅱ级(P<0.05)。TIMP-4在正常子宫颈、原位癌及浸润性癌组织中的表达呈下降趋势,但差异无显著性(P>0.05);TIMP-4的表达与子宫颈癌的临床分期有密切关联,其在早期癌中的表达高于晚期癌(P<0.05)。MMP-26与TIMP-4在子宫颈癌组织中的表达呈负相关关系(rs=-0.259,P<0.05);MMP-26与MMP-9蛋白在子宫颈癌组织中的表达无相关性(rs=0.140,P>0.05)。结论:MMP-26和MMP-9在宫颈癌组织中高表达,促进其侵袭和转移;TIMP-4在子宫颈癌组织中的表达下降,提示三者联合应用有望成为一项判断子宫颈癌预后的指标。

Objective To detect the expressions of matrix metalloproteinase-26（MMP-26）,metalloproteinase-9（MMP-9） and tissue inhibitors of metalloproteinase-4（TIMP-4） in the cervical carcinoma tissues and analyze their correlation with clinical features.Methods The expressions of MMP-26,MMP-9 and TIMP-4 were detected in 10 cases of normal cervical tissues,6 cases of cervical carcinoma in situ and 79 cases of invasive carcinoma of cervix by immunohistochemistry.Results MMP-26 showed strongly positive staining in cervical carcinoma in situ and invasive carcinoma of cervix,their positive rates were 83.33% and 82.47%,respectively;there were significant differences compared with normal cervix（P〈0.05）.The expression of MMP-26 was closely correlated with the depth of myometrial invasion and lymph node metastasis（P〈0.05）.The positive rate of MMP-9 in cervival carcinoma tissue was 68.35%.The expression of MMP-9 was strongly correlated with pathological grading,its expression in grade Ⅲ was significantly higher than those in grade Ⅰ and Ⅱ（P〈0.05）.The expression of TIMP-4 gradually decreased from normal cervical tissue to carcinoma in situ and invasive carcinoma,but no significant difference（P〉0.05）.The expression of TIMP-4 was strongly correlated with clinical stage,its expression in early stage was significantly higher than that in late stage（P〈0.05）.The expression of MMP-26 showed negative correlation with TIMP-4（rs=-0.259,P0.05）,while there was no correlation between MMP-26 and MMP-9（rs=0.140,P〈0.05）.Conclusion The expressions of MMP-26,MMP-9 are increased in cervix carcinoma tissue.They promote the local invasion and metastasis of cervix carcinoma tissue.The expression of TIMP-4 is decreased in cervix carcinoma tissue.Their application together could be a marker to monitor the prognosis of vervical carcinoma tissue.