萝卜优异种质对芜菁花叶病毒抗性的遗传分析

Genetic Analysis of the Resistance to TuMV in Elite Radish Germplasm

为了深入认识萝卜优异种质对芜菁花叶病毒(TuMV)的抗性遗传机制,本研究以高抗TuMV的自交系Q07-12(P_1)和高感自交系KB07-10(P_2)为亲本,构建了包括P_1、P_2、F_1、B_1、B_2及F_2的6个群体,采用数量性状主基因+多基因混合遗传的六世代联合分析法,对其抗性遗传规律进行了研究。结果表明,萝卜对TuMV的抗性受两对主基因控制,并符合"加性-显性-上位性主基因+加性-显性-上位性多基因"模型,其中主基因遗传率在B_1、B_2及F_2群体中分别为89%、55%和95%,多基因的遗传率分别为0、40.9%和0,环境因素对抗性强弱的影响较小。此结果表明了该优异种质在萝卜抗病毒遗传改良中的利用价值,为萝卜抗病育种和抗病基因的挖掘提供了理论依据。

In order to further understand the genetic mechanism on the resistance to turnip mosaic virus （TuMV）in elite radish germplasm, the inheritance was analyzed by applying the joint segregation analysis of a mixed genetic model of major gene plus polygene in six generations（ Pl, P2, F1, B1, B2 and F2 ） from the cross between highly resistant inbred line Q07-12（ PI ）and highly susceptible line KB07-10（ P2 ）. The inheritance of resistance to TuMV was fitted by the two major genes plus polygenes mixed model（ E model）. In B1 ,B2and F2populations, the estimated heritability of major genes were 89% ,55% and 95 % , respectively. The heritability of polygenes were 0,40.9% and 0 respectively. The environmental factors had small effect on the resistance to TuMV in radish. These results indicate that the elite resistant radish germplasm is of value in radish genetic improvement, and provide the genetic basis for improving the efficiency of breeding for TuMV-resistance.