小麦根蛋白提取与双向电泳方法的优化与应用

Optimization and Application on Two-Dimensional Electrophoresis for Wheat Root Proteomic Study

为了建立一套适合小麦根蛋白质组分析的双向电泳系统(2-DE),得到更加清晰的电泳图谱,本研究以小麦幼苗根系为材料,对根蛋白的提取方法、上样量等进行了优化。研究发现,上样量为1200μg时,Trizol抽提法提取的蛋白能够获得图像清晰、分辨率高、重复性好的双向电泳图谱,基本满足小麦根系蛋白质组学的分析和研究。

To develop an efficient and high resolution 2-DE procedure for wheat root proteomic analysis, the method of wheat root protein extraction and sample preparation for two-dimensional gel electrophoresis（2-DE）were optimized by using the roots of wheat euhivars Jing-411 and Chinese Spring at two leaves stage. Separation results of wheat root protein by using the optimized method showed that better resolution of the electrophoresis patterns could be achieved. In particular,the protein samples extracted from Trizol reagent appeared to be better for two-dimensional gel electrophoresis separation,which could be used as an important tool for the studies on the wheat root proteome.