中西医结合抗绿脓杆菌生物膜作用的研究

EFFECT OF ANTI-PSEUDOMONAS AERUGINOSA BIOFILM BY COMBINING CHINESE AND WESTERN MEDICINE

研究了金银花水煎液体外抗铜绿假单胞菌生物膜的作用及与庆大霉素的协同作用:通过试管稀释法测定了金银花水煎液和庆大霉素对铜绿假单胞菌的最小抑菌浓度(mini mal in-hibitory concent ration,MIC),用MTT法建立了体外铜绿假单胞菌生物膜并检定了金银花水煎液和庆大霉素不同MIC浓度的抗铜绿假单胞菌生物膜的作用.结果表明:金银花水煎液对绿脓杆菌的MIC为125 mg/mL,SMIC也为125 mg/mL;庆大霉素对绿脓杆菌的MIC为1.56μg/mL,SMIC为25μg/mL,与金银花的协同作用对绿脓杆菌的SMIC为1.56μg/mL.由实验结果可知金银花水煎液在体外能抑制铜绿假单胞杆菌(Pseudomonas aeruginosa,P.A)对固体表面的黏附能力及生物膜(biofil m,BF)形成能力,并能破坏P.A已形成的BF,增强庆大霉素对BF内铜绿假单胞菌的清除作用.

To observe the effect of anti-Pseudomonas aeruginosa biofilm by honeysuckle decoction and the role of the synergies with gentamicin. By test-tube dilution, the minimum inhibitory concentration （MIC） of honeysuckle decoction and gentamicin against Pseudomonas aeruginosa have been determined. The Pseudomonas aeruginosa biofilm has been ob- tained in vitro by MTT method and the role of anti-Pseudomonas aeruginosa biofilm with different MIC concentrations of honeysuckle decoction and gentamicin have been tested. The MIC of honeysuckle decoction against Pseudomonas aeruginosa is 125 mg· mL^-1 and the SMIC is also 125 mg · mL^-1. To gentamicin, the MIC is 1.56 μg · mL and the SMIC is 25 μg ·mL^-1. The SMIC of honeysuckle decoction with gentamicin is 1. 56 μg ·mL^- 1. From those result we can see honeysuckle decoction can inhibit the adhesion of P. a to solid surface and the forming ability of BF. Moreover, damage the BF produced by P. a and enhance the scavenging of BF by gentamicin.