东亚钳蝎毒素ITX对C57BL/6小鼠Lewis肺癌组织生长的影响

The Effects of ITX from Buthus Martensii Karsch on the Growth of Lewis Lung Carcinoma in C57BL/6 Mice

目的观察东亚钳蝎毒素ITX对C57BL/6小鼠Lewis肺癌(LLC)组织生长的影响。方法32只C57BL/6小鼠随机分为空白组、模型组、阳性对照rIL-2组和ITX组。将LLC细胞接种至模型组、阳性对照rIL-2组和ITX组C57BL/6小鼠的右前腋下。各组按不同的处理因素连续干预16 d。实验期间观察各组小鼠的一般状态,监测瘤重和瘤体积的变化。末次给药24 h后处死小鼠,剥取肿瘤,摘取双肺叶,在光学显微镜下观察肿瘤和肺脏的炎性细胞浸润情况。结果与模型组相比,ITX组和rIL-2组小鼠的生存质量有一定程度的下降,但差异无统计学意义(P>0.05);各组肿瘤体积比较差异无统计学意义(P>0.05);肿瘤间质和肺泡间隔的炎细胞数增多(P<0.05)。随着时间延长,模型组、ITX组和rIL-2组肿瘤体积均有明显增长(P<0.05)。结论ITX能够通过加强肿瘤微环境中以炎症为代表的非特异免疫促进C57BL/6小鼠LLC的生长。

Objective To study the effects of ITX on the growth of Lewis lung carcinoma（LLC） in C57BL/6 mice.Methods 32 C57BL/6 mice were divided at random into blank group,model group,rIL-2 group and ITX group.LLC cells were inoculated into the right upper limbs of C57BL/6 mice in the model group,the rIL-2 group and the ITX group.All the groups were treated for continuous 16 days by different factors.During this period,all the mice＇s common state,tumor weight and volume were observed.Twenty four hours after the last administration,all the mice were sacrificed,and their tumors and double-lungs were removed.The infiltrative state of inflammatory cells in tumor or lung was observed under the light microscope.Results As compared with the model group, the life quality of the rIL-2 group and the ITX group was decreased in a certain degree（P〉0.05）,and the tumor volume of the rIL-2 group and the ITX group increased in some degree（P〉0.05）.Inflammatory cells were higher than those in the model group in interstitial substance of tumor or alveolar septum in the ITX and the rIL-2 group（P〈0.05）.With the extension of time,the tumor volume of the model group,the rIL-2 group and the ITX group increased evidently（P〈0.05）.Conclusion ITX could facilitate the growth of LLC in C57BL/6 mice through strengthening non-specific immunity represent inflammatory reaction.