新型神经保护剂TQ0701-2对大鼠脑缺血再灌注损伤的保护作用

Protective effect of TQ0701-2 on cerebral ischemia reperfusion injury in rats

目的:研究新型神经保护剂TQ0701-2对大鼠脑缺血再灌注损伤的保护作用。方法:将120只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(3.0mg/kg)以及TQ0701-2高剂量组(6.0mg/kg)、中剂量组(3.0mg/kg)、低剂量组(1.5mg/kg)。假手术组仅进行手术而不造成缺血状态,其余各组均采用Longa线栓法制备大鼠MCAO模型,在缺血2h后进行再灌注。TQ0701-2三个剂量组和依达拉奉组分别在缺血前30min以及再灌注0、2h尾静脉注射TQ0701-2和依达拉奉,假手术组和模型组则给予等量的生理盐水。再灌注24h后观察大鼠神经功能损伤症状、脑组织梗死率以及病理组织学的改变。结果:模型组大鼠神经功能损伤严重,脑组织梗死率也明显增高(P<0.01vs假手术组)。与依达拉奉的保护作用相同,TQ0701-2高中低三个剂量均能显著降低MCAO大鼠的神经功能评分和脑组织梗死率(P<0.01vs模型组),并且三个剂量的改善作用是随着浓度增大而增强的,具有剂量相关性。另外,TQ0701-2对大鼠脑缺血再灌注所致的神经元变性、坏死也有一定的保护作用。结论:研究表明,依达拉奉衍生物TQ0701-2对大鼠的脑缺血再灌注损伤有明显的神经保护作用。

AIM： To investigate the protective effect of a new type nerves protectant TQ0701- 2, as the derivate of edaravone free radical scavenger, on cerebral ischemia reperfusion injury rat. METHODS： 120 mate SD rats were randomly divided into sham group, model group, edaravone group （3.0 mg/kg） and TQ0701-2 high- dose group （ 6.0 mg/kg）, middle-dose （ 3.0 mg/kg） and low-dose group （1.5mg/kg）. Animals in the latter five groups were subjected to transient focal ischemia by the middle cerebral artery occlusion （MCAO） for 2 h before reperfusion, while the sham group wasn＇t ischemic. The rats were injected with edaravone or TQ0701-2 30 min before ischemic and 0 min, 2 h after reperfusion in edaravone and TQ0701-2 groups, sham group and model group were treated with normal saline as well. After 24 h of reperfusion, the infarct ratio, neurological and histological deficient and the changes of pathohistology were evaluated. RESULTS. The infarct ratios and neurological deficit scores in the model group were increased （P（0.01 vs. sham group）. The neurological deficit scores and infarct ratios in three doses of TQ0701-2 were significantly decreased （P〈0.01 vs. model group）, similar to edaravone, and the protection was enhanced in a dose-dependent manner. Moreover, TQ0701-2 improved neuronal degeneration and necrosis induced by ischemia reperfusion. CONCLUSION： These results show that the the derivate of edaravone TQ0701-2 has notable protective effect on cerebral ischemia reperfusion injury in rats.