摘要
目的探讨食管鳞癌组织中磷酸化蛋白激酶B(P-AKT)的表达及其临床相关性。方法应用免疫组织化学和蛋白免疫印迹方法检测120例食管鳞癌及远癌旁正常食管黏膜组织中P-AKT蛋白的表达情况。结果P-AKT蛋白在食管鳞癌中表达阳性率(70.8%)明显高于相应远癌旁正常食管黏膜组织(20%)(P<0.01);其表达与淋巴结转移相关(P<0.05),与浸润深度、分化程度无关(P>0.05)。结论P-AKT蛋白的过表达可能在食管鳞癌的发生、发展及转移中起重要作用且可能成为靶向治疗的新靶点。
Objective To investigate the expression of P-AKT protein and their relationship with clinic characteristics of esophageal squamous cell carcinoma（ESCC）.Methods Expression of P-AKT protein was examined by Western blot and immunohistochemical method in the ESCC and normal esophageal（control）tissue of 120 patients.Results The positive rate of P-AKT was significantly higher in ESCC（70.8%）than in the control tissue（20%）（P〈0.01）;and expression level of P-AKT was significantly correlated with lymphnode metatasis（P〈0.05）;negatively correlation with histological differentiation and depth of invasion（P〉0.05）.Conclusion The hyperexpression of P-AKT may play an important role in the initiation and devolopment of human ESCC,and it may be a new target for targeted therapy of ESCC.
出处
《安徽医科大学学报》
CAS
北大核心
2010年第1期24-27,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金-973项目(编号:2004CB518604)
关键词
食管肿瘤
癌
鳞状细胞
免疫组织化学
印迹法
蛋白质
esophageal neoplasms
carcinoma
squamous cell
immunohistochemistry
blotting
western
