类风湿关节炎患者血清内皮抑素、碱性成纤维细胞生长因子、抗环瓜氨酸肽抗体的联合检测与应用

The application of combined detection of endostatin,bFGF and the anti-cyclic citrullinated peptide antibody in patients with rheumatoid arthritis

目的检测类风湿关节炎(RA)患者血清中内皮抑素(endostatin)、碱性成纤维细胞生长因子(bFGF)和抗环瓜氨酸肽(anti-CCP)抗体水平,并探讨其在RA中的临床意义。方法选取RA患者30例,健康对照30例。用酶联免疫吸附法(ELISA)检测患者血清中endostatin、bFGF和anti-CCP抗体水平。结果①RA患者血清中endostatin的水平(62.57±24.96)ng/ml,明显高于健康对照组(43.59±8.58)ng/ml(P<0.001),bFGF的水平(24.46±11.50)pg/ml明显高于健康对照组(16.07±4.12)pg/ml(P<0.002),RA患者血清中anti-CCP(17.44±4.35)RU/ml也明显高于健康对照组(2.32±0.97)RU/ml(P<0.001)。②相关性分析显示en-dostatin水平与bFGF呈高度正相关(r=0.934,P<0.01),endostatin水平与anti-CCP抗体呈高度正相关(r=0.836,P<0.01),bFGF与anti-CCP抗体(r=0.789,P<0.01)具有中度相关性。结论endostatin、bFGF及anti-CCP抗体对RA具有较好的敏感性和高度的特异性,联合检测endostatin、bF-GF、anti-CCP抗体可作为RA患者及RF阴性RA患者的诊断指标。

Objective To detect endostatin,basic fibroblast growth factor（bFGF）and the anti-cyclic citrullinated peptide antibody（anti-CCP）in rheumatoid arthritis（RA）and to explore its value in the pathogenesis of RA.Methods Serum samples were obtained from 30 RA patients,30 healthy individuals.Serum level of endostatin,bFGF and anti-CCP were examined by enzyme-linked immunosorbent assay（ELISA）.Results ①The concentration of endostatin in RA（62.57±24.96）ng/ml was significantly higher than that in healthy controls（43.59±8.58）ng/ml（P〈0.001）.The concentration of bFGF in RA（24.46±11.50）pg/ml was significantly higher than that in healthy controls（16.07±4.12）pg/ml（P〈0.002）.The concentration of anti-CCP in RA（17.44±4.35）RU/ml was significantly higher than that in healthy controls（2.32±0.97）RU/ml（P〈0.001）.②There were highly positive correlations between endostatin and bFGF（r=0.934,P0.01）,endostatin and anti-CCP antibody（r=0.836,P0.01）,bFGF and anti-CCP antibody（r=0.789,P0.01）.Conclusion Endostatin,bFGF and anti-CCP are quite sensitive and specific in the diagnosis of RA.To detect the endostatin,bFGF and anti-CCP may be useful factors for positive or negative RA patients.