无精子症患者睾丸细针穿刺后行倒置显微镜检查与传统病理组织切片检查精子检出率的比较

The comparison of the testicular tissue sperm recovery rates detected by using inverted microscopy and traditional histopathological examination after testicular fine needle aspiration

目的探讨倒置显微镜检查与传统病理组织切片检查对睾丸细针抽吸术(TEFNA)所取睾丸组织的精子检出率。方法90例无精子症患者接受诊断性TEFNA。将取出的每份睾丸曲细精管分别行:撕碎后制成细胞悬液于倒置显微镜下检查有无精子和传统病理组织切片检查有无精子,比较两种方法的精子检出率。结果90例无精子症患者行TEFNA获取的睾丸组织,制成细胞悬液于倒置显微镜下检出有精子者为68例(75.6%);病理组织切片检查检出有精子者为55例(61.1%)。结论TEFNA是一种简单、有效的获取无精子患者睾丸组织的方法,对于所获取的睾丸组织通过撕碎后倒置显微镜检查精子检出率优于传统病理组织切片精子检出率,且具有耗时短、费用少的优点。

Objective To compare the testicular tissue sperm recovery rates by using inverted microscopy and traditional histopathological examination.Methods Testicular fine needle aspiration（TEFNA）was performed in 90 azoospermic men to obtain the testicular tissue.Then the tissue was divided into two groups.In group Ⅰ,a half of the tissue was added into a droplet and minced into single cells with two syringes,then detected the sperm recovery rate with an inverted microscopy.For group Ⅱ,the rest tissue was detected by traditional hitstopathological examination according to Johnsens score criterion.Categorical data were analyzed with χ^2 test.Results In group I,sperm were found in 68 cases using inverted microscopy among the 90 men,while in group Ⅱ,only 55 cases recovered sperm by the traditional hitstopathological examination.The sperm recovery rate in group Ⅰ was significantly higher than that in group Ⅱ（75.6% vs 61.1%,P〈0.05）.Moreover,less time was used in recovering sperm in group Ⅰ than in group Ⅱ.Conclusion Our date indicate that inverted microscopy examination of spermatid cell suspension is an quick,simple method to evaluate the testicular tisse,and can achieve a higher sperm recovery rate compare with the traditional hitstopathological examination for azoospermic patients.