肝细胞永生化：实验研究与应用

Immortalization of liver cells： experimental studies and appli- cation

肝细胞是生物人工肝支持系统的核心原材料，原代肝细胞由于体外生存期短、培养困难而限制了它的应用。目前常用的猪肝细胞有感染内源性逆转录病毒之忧。近年来随着分子生物学的发展肝细胞永生化已成为可能。肝细胞的永生化对于药物毒理、生物人工肝支持系统以及肝脏组织工程的研究都具有非常深远的意义。本文就肝细胞永生化的研究现状及应用前景作一综述。

Hepatocyte is the core raw materials of bioartificial liver support system, primary hepatocyte is lim- ited to application because of short survival and difficult cul- ture in vitro. Porcine hepatocyte which has been used re- cently exist the risks of endogenous retrovirus transmission. With the development of molecular biology, it has been pos- sible that hepatocyte is immortalized recently. Immortalized hepatocytes have greatly significant to drug toxicology, bio- artificial liver support system and tissue engineering of liver. Therefore, we will review the prospects for research and application of immortalized hepatocytes.