糖尿病大鼠甲状腺组织RAGE mRNA和蛋白表达的变化

Changes of RAGE mRNA and Protein Expression in Thyroid of Diabetic Mellitus Rats

目的:探讨糖尿病(DM)大鼠甲状腺组织终末糖基化产物受体(RAGE)mRNA和蛋白的表达水平及其与血糖的关系。方法:经高脂饲料喂养联合注射小剂量链脲佐菌素制备DM大鼠49只,随机分为DM组(17只)、胰岛素干预组(DI组,16只)、氨基胍干预组(DA组,16只),另设正常对照组(NC组,9只)。成模12周末测空腹血糖后,经股动脉放血处死大鼠。取甲状腺组织,分别以GAPDH和β-actin作为内对照,用半定量RT-PCR和WesternBlot方法测定各组RAGEmRNA和蛋白的表达。结果:DM组大鼠血糖为(31.72±9.29)mmol/L、甲状腺组织RAGE mRNA与GAPDH mRNA表达比为0.90±0.30,RAGE蛋白与β-actin蛋白表达比为0.88±0.23,均高于NC组的(4.87±0.75)mmol/L、0.27±0.17和0.44±0.21,差别有统计学意义(均P<0.05),DI组与DA组大鼠甲状腺组织RAGEmRNA和蛋白表达分别为0.60±0.22、0.67±0.27和0.38±0.20、0.45±0.17,均低于DM组(均P<0.05)。结论:DM大鼠甲状腺组织RAGE mRNA和蛋白表达增高;积极地控制血糖、阻断终末糖基化产物的形成有利于抑制甲状腺组织RAGE高表达。

Objective：To investigate the expression of receptor for advanced glycation end products （RAGE） in the thyroid tissue of diabetes mellitus （DM） rats.Methods：The rat model of DM was established by high-lipid diet and vena caudalis injection of streptozocin.The rats of DM models were then randomly divided into three groups：DM group（n=17）,insulin intervention group（n=16） and aminoguanidine intervention group （n=16）.Nine normal rats were taken as controls.Twelve weeks after the establishment of DM model,rats were sacrificed and the thyroid tissue was taken to determine RAGE mRNA and the protein expression with the method of RT-PCR and Western Blot.Results：The levels of RAGE mRNA and protein expression were higher in the thyroid tissue of DM rats than those in controls （P 0.05）.The levels of RAGE mRNA and protein expression were lower in the thyroid tissue of insulin intervention group and aminoguanidine intervention group than those of DM group （P 0.05）.Conclusion：The levels of RAGE mRNA and protein expression were up-regulated in the thyroid tissue of DM rats,which can be restrained by controlling blood glucose and blocking the formation of advanced glycation end products.