汉防己甲素对联苯苄唑抗须癣毛癣菌活性增效作用的体内实验研究

Experiment on Tetrandrine as A Synergist to Bifonazole Against Trichophyton mentagrophytes in vivo

目的研究汉防己甲素(tetrandrine,TET)对联苯苄唑(bifonazole,BFZ)治疗须癣毛癣菌所致豚鼠皮肤癣菌病是否有增效作用。方法以须癣毛癣菌构建豚鼠皮肤癣菌病模型,将造模成功的20只豚鼠按随机数字表法等分为4组,进行双盲实验,分别涂用复方BFZ(含1%BFZ和1%TET)、1%BFZ、1%TET和基质,1次/d,共14天,进行皮损评分、病原学和病理学检查,分析4组药物间疗效差异。结果用药期间,4组皮损评分差异有统计学意义(F=93.49,P=0.000);从用药第3天起,复方BFZ组和1%BFZ组皮损评分逐日下降,前者皮损评分持续低于后者,差异有统计学意义(P=0.000);1%TET组和基质组皮损评分差异无统计学意义(P=1.000)。复方BFZ组皮损真菌学检查比1%BFZ组转阴早2天,炎症反应轻于其他3组。结论TET对BFZ治疗须癣毛癣菌所致豚鼠皮肤癣菌病有增效作用。

Objective To investigate the synergistic effect of tetrandrine （TET） to bifonazole（BFZ） treatment of dermatophytosis in guinea pigs infected with Trichophyton mentagrophytes. Methods Establishing the dermato- phytosis model in guinea pigs with Trichophyton mentagrophytes infection. The 20 infected animals were randomly and equally divided into 4 groups to conduct double-blind experiment and were applied with BFZ compound cream （ containing 1% BFZ and 1% TET）, 1% BFZ cream, 1% TET cream and matrix cream once per day for successive 14 days respectively. The alterations in the skin lesion scores, mycological and pathology were observed to analyze the efficacy differences between four groups of drugs. Results During the treatment, there were obvious statistical difference （F = 93.49, P = 0. 000）between the skin lesion scores in the four groups; the skin lesion scores of BFZ compound cream group and 1% BFZ cream group started to fall gradually from the 3th day, the former was significantly more effective than the latter, with obviously statistical difference （P =0. 000） ; there was no statistical difference （P = 1. 000） between the skin lesion scores of 1% TET cream group and matrix cream group. In the BFZ compound cream group, the skin lesion scores became negative 2 days earlier than the 1% BFZ cream group, and inflammatory reaction was slighter than the other 3 groups. Conclusion There is synergistic effect of TET on BFZ treatment of dermatophytosis in guinea pigs infected with Trichophyton mentagrophytes.