吸入一氧化碳抗肢体缺血/再灌注损伤的实验研究

Carbon monoxide inhalation protects against limb ischemia/reperfusion injury

目的:观察吸入适量一氧化碳(CO)对大鼠肢体缺血/再灌注(I/R)损伤的防治作用。方法:SD大鼠44只,随机分为假手术(S)、I/R、I/R吸入CO(RC)组;通过夹闭股动脉4h、再开放48h,复制肢体I/R损伤模型;RC组行再灌注时,使动物吸入含有CO的医用空气(CO的体积分数为0.05%),其余两组呼吸正常空气;对比观测缺血肢体大体及骨骼肌组织病理学、缺血肢体湿干重比值(W/D)的变化,流式细胞仪检测肌组织中Bax、Bcl-2的表达水平及细胞凋亡百分比,全自动生化分析仪检测血清乳酸脱氢酶(LDH)和肌酸激酶(CK)的变化。结果:与I/R组比较,RC组动物W/D、血清LDH及CK含量、肌组织中Bax表达水平及细胞凋亡百分比均显著降低,肌组织Bcl-2表达水平显著升高,缺血肢体大体观及肌组织病理学明显改善。结论:吸入适量浓度的外源性CO对肢体I/R损伤有防治作用。

Objective：To study the protective effect of carbon monoxide（CO） inhalation in the limb ischemia/reperfusion（I/R） injury of rats.Methods：Forty-four Sprague-Dawley rats were randomly divided into three groups：S,I/R and RC groups.I/R injury model was made by the occlusion of the femoral artery for four hours and the reperfusion for forty-eight hours.RC group was exposed to medical air mixed CO （the volume fraction of CO：0.05%） during limb reperfusion in rats.The other two groups were exposed to the routine air.Gross morphology of the ischemic limb,wet-to-dry weight ratio （W/D）,and skeletal muscle histopathology were observed.The apoptosis index and expression levels of Bax and Bcl-2 in the muscle were assessed with Flow Cytometry.The activities of serum lactate dehydrogenase （LDH） and creatine kinase （CK） were tested by Automatic Biochemical Analyzer.Results：Compared to I/R group,W/D,serum LDH and CK activities,the apoptosis index and Bax expression level in the muscle were all significantly decreased,the Bcl-2 expression level was significantly increased,gross morphology of the ischemic limb and muscle histopathology were obviously improved in RC group.Conclusion：Inhaling exogenous CO can attenuate limb I/R injury.