长期异相睡眠剥夺对大鼠能量代谢及血清甲状腺素水平的影响

The effects of chronic rapid eye movement sleep deprivation on energy metabolism and FT3,FT4 level in serum of rats

目的:探讨长期异相睡眠剥夺对大鼠能量代谢及血清甲状腺素水平的影响。方法:采用小平台水环境法建立长期异相睡眠剥夺大鼠模型;检测其能量代谢变化;放射免疫法检测血清中甲状腺素水平。结果:睡眠剥夺后大鼠摄食量由(75.06±25.37)g/(d.kg)增加到(122.30±20.43)g/(d.kg),体重由(360.89±43.01)g减轻到(295.97±37.95)g,体温由(37.62±1.12)℃先升高到(39.00±0.87)℃后又降低至(37.72±0.84)℃,基础代谢率由(1.69±0.36)mlO2/(g.h)增加到(2.40±0.09)mlO2/(g.h)与对照组相比差异显著(P<0.05);血清中游离三碘甲状腺原氨酸(FT3)水平由(3.38±0.88)pmol/L降低到(2.38±0.83)pmol/L,游离甲状腺素(FT4)由(14.62±3.62)pmol/L降低到(8.26±2.80)pmol/L与对照组相比差异显著(P<0.05)。结论:长期异相睡眠剥夺可以显著影响大鼠的能量代谢和血清甲状腺素水平。

Objective：To study the effect of chronic rapid eye movement sleep deprivation on energy metabolism,FT3,FT4 in serum.Methods：Rapid eye movement sleep deprivation of rats were deprived by flower pot,and then the energy metabolism were detected.The FT3,FT4 level in serum was determined by radioimmunoassay kit.Results：Rats after sleep deprivation displayed food intake increased from（75.06±25.37）g/（d·kg） to（122.30±20.43）g/（d·kg）,body weight substantially decreased from（360.89±43.01）g to （295.97±37.95）g,body temperature from （37.62±1.12）℃ up to the first （39.00±0.87）℃ and then reduced to （37.72±0.84）℃,the basal metabolism rate increased significantly from（1.69±0.36）mlO2/（g·h） to （2.40±0.09）mlO2/（g·h）,compared with the control group（P〈0.05）.Sleep deprivation also resulted significantly lower serum thyroxine levels in comparison with the control,serum free triiodothyronine（FT3） level reduced from （3.38±0.88）pmol/L to （2.38±0.83）pmol/L,then free thyroxine（FT4） decreased from （14.62 ± 3.62）pmol/L to （8.26±2.80）pmol/L（P〈0.05）.Conclusion：Rapid eye movement sleep deprivation can change energy metabolism remarkable,as well as the alteration of FT3,FT4 levels in serum.