三七总皂苷对家兔离体小肠平滑肌自发收缩活动的影响

Influences of Panax notoginsenosid on spontaneous contraction of small intestine smooth muscle of rabbits in vitro

目的:观察三七总皂苷对家兔离体小肠平滑肌收缩活动的影响,并探讨其作用机制。方法:取健康家兔,雌雄不拘,将小肠离体后恒温灌流,观察三七总皂苷对家兔小肠自发收缩活动的影响;在灌流液中分别加入BayK8644、左旋硝基精氨酸甲酯(L-NAME)后再加入三七总皂苷,研究其作用机制;在无钙台式液中加入rynodine后再加入三七总皂苷,研究其作用机制。结果:三七总皂苷剂量依赖性的抑制家兔离体小肠平滑肌收缩的幅度。BayK8644和L-NAME均可完全阻断三七总皂苷对家兔小肠平滑肌收缩活动的抑制作用。在无钙台式液中,三七总皂苷显著抑制rynodine引起的细胞内钙收缩活动。结论:三七总皂苷显著抑制家兔小肠平滑肌的收缩活动,其抑制收缩活动机制可能是:增加小肠平滑肌NO浓度,从而抑制细胞外钙内流和内钙释放。

Objective：To observe the influences of Panax notoginsenosid（a compound of Chinese Traditional Medicine） on the spontaneous contraction of small intestine smooth muscle of rabbits in vitro and explore the mechanism.Methods：The influences of Panax notoginsenosid on the spontaneous contraction of small intestine in intacted rabbits（male or female） after the isothermal perfuse of small intestine in vitro were observed.Bay K8644 and nitro-L-arginine methylester（L-NAME） were added to the normal Tyrode＇s solution respectively before Panax notoginsenosid.In the Ca^2＋ free Tyrode＇s solution,rynodine was added before Panax notoginsenosid.The mechanism of Panax notoginsenosid was studied.Results：Panax notoginsenosid reduced the amplitude of contraction of small intestine smooth muscle in rabbits in a does-depended manner.Bay K8644 and L-NAME could completely block the inhibition of Panax notoginsenosid on the contraction of small intestine smooth muscle.Panax notoginsenosid inhibited significantly the intracellular calcium-depended contraction induced by rynodine in the Ca^2＋ free Tyrode＇s solution.Conclusion：Panax notoginsenosid inhibits significantly the contraction of small intestine smooth muscle of rabbits in vitro.The mechanism may be related to increase NO concentration in small intestine smooth muscle so that inhibit extracellular Ca^2＋ inflo-wing via cell membrane and intracellular Ca^2＋ releasing via sarcoplasmic reticulum.