内质网应激对γ射线照射诱导肿瘤细胞死亡的影响

Effect of endoplasmic reticulum stress on γ-ray irradiation-induced tumor cell death

目的探讨内质网应激反应下肿瘤细胞对辐射敏感性的影响。方法将人宫颈癌HeLa细胞置于60Co设备行γ射线照射;采用反转录PCR方法检测内质网应激反应标志分子X盒结合蛋白1(XBP1)mRNA剪切;细胞克隆形成试验检测内质网应激反应剂二硫苏糖醇(DTT)作用后对HeLa细胞的生存变化。结果DTT作用后XBP1 mRNA出现剪切;克隆形成试验发现HeLa细胞存活率明显提高。结论在内质网应激反应下,增加肿瘤细胞的辐射抵抗性。

Objective To investigate the effect of endoplasmic reticulum stress on sensitivity of tumor cells to radiation.Methods HeLa cells were irradiated with 60Co γ-ray irradiation.We used reverse transcription-PCR method to detect mRNA splicing of X-box binding protein 1（XBP1）,a marker of endoplasmic reticulum stress response;survival changes of HeLa cells under endoplasmic reticulum stress response were assayed by clonogenic survival assay.Results After DL-dithiothreitol treatment,XBP-1 mRNA splicing of HeLa cells were performed,survival fraction of HeLa cells were increased by clonogenic survival assay with DTT treatment.Conclusion Endoplasmic Reticulum Stress increases resistance of tumor cell in radiation.