实时荧光PCR和HC-Ⅱ检测高危型人乳头瘤病毒的临床价值被引量：1

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摘要目的评价实时PCR和第2代杂交捕获法(HC-Ⅱ)对不同宫颈病变高危型人乳头瘤病毒(HPV)检测的临床价值。方法采用实时荧光PCR与HC-Ⅱ检测158例宫颈病变患者宫颈脱落细胞中的高危型HPV,比较两者的检测结果。结果HC-Ⅱ和PCR检测高危型HPV的阳性率分别为69.0%和72.8%,二者总符合率85%(134/158);两种方法检测高度鳞状上皮细胞病变的阳性率均明显高于炎症(P均<0.05);其中115例PCR阳性标本中,HPV16阳性率为31.3%,HPV18为11.3%,HPV16、18均阳性为12.2%。结论PCR与HC-Ⅱ检测高危型HPV有较好的相关性;HPV-DNA检测可作为预防和早期诊断子宫颈癌的主要筛查手段;PCR检测HPV16/18可作为宫颈癌筛查经济、有效的方法。

作者沈云岳刘华王蕾高锋

机构地区上海交通大学附属第六人民医院

出处《山东医药》 CAS 北大核心 2010年第5期77-78,共2页 Shandong Medical Journal

关键词实时荧光聚合酶链反应第2代杂交捕获法人乳头瘤病毒宫颈上皮内瘤样变

分类号 R737.33 [医药卫生—肿瘤]

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参考文献5

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