中药干预模拟失重大鼠股骨Ⅰ型胶原表达的研究

Effect of a Chinese herbal prescription on collagen Ⅰ in rat's femur under simulated weightlessness

目的:研究3周模拟失重对大鼠股骨Ⅰ型胶原表达的影响和中药复方的干预作用。方法:雄性Wistar大鼠,30只,随机分为空白组、悬尾模型组和悬尾中药组;后两组不悬吊适应1周,悬尾吊3周模拟失重,中药组予中药复方(含熟地黄、怀牛膝、黄芪、当归、牡蛎醋酸水解物等)水煎剂灌胃。实验结束取左股骨,免疫组化法观察股骨颈骨组织中Ⅰ型胶原表达。结果:经过3周尾吊模拟失重,与空白组比较,模型组股骨Ⅰ型胶原阳性计数和IOD值均出现显著下降(P<0.001);中药组股骨Ⅰ型胶原阳性计数和IOD值较空白组变化不显著(P>0.05),较模型组则有显著增加(P<0.001)。结论:3周模拟失重可造成模型动物股骨Ⅰ型胶原蛋白合成严重障碍,受试中药复方对此有显著的改善作用。

Objective：To investigate the effect of a Chinese herbal prescription on collagenⅠ in rat＇s femur under simulated weightlessness. Methods：Thirty Wistar rats were randomly divided into 3 groups：blank control group （10 rats）,tail suspension group （TS,10 rats）,TS with Chinese medicine group （10 rats）. Rats in TS with Chinese medicine group took a Chinese herbal prescription （contains Radix Rehmanniae Praeparata,Radix Achyranthis Bidentatae,Radix Astragali,Radix Angelicae Sinensis,Concha Ostreae prepared by acetic acid）by oral administration. After 1 week adaption and 3 weeks tail suspending,rat＇s left femur was colleced,and collagenⅠ in femur neck was detected by immunohistochemical method. Results：Counts and integral optical density（IOD） of collagenⅠcoloration decreased significantly in TS group（P〈0.001）,but no significant change in TS with Chinese medicine group （P〉0.05）,as compared with control group. Conclusion：Generation of collagen I become weaken under simulated weightlessness,while the Chinese herbal prescription is effective to prevent the change,thus biochemistry environment of bone calcium deposition may be improved by this Chinese herbal prescription under simulated weightlessness.