免疫磁珠分离涂片和PCR检测痰内结核分枝杆菌的诊断价值研究

The diagnosis value of immunomagnetic bead separation of mycobacterium tuberculosis from sputum for smear and polymerase chain reaction

目的:探讨免疫磁珠分离涂片染色镜检法和免疫磁珠分离-PCR法对痰标本中结核分枝杆菌的诊断价值。方法:利用纯化的兔抗H37Rv血清包被磁珠,对痰中结核分枝杆菌进行分离,再进行涂片和PCR检测。结果:免疫磁珠分离涂片法比直接涂片法(χ2=40.83,P=0.039)、免疫磁珠分离-PCR法比常规PCR(χ2=23.31,P<0.01)的灵敏度高。结论:免疫磁珠分离涂片法和磁珠分离-PCR法因操作简单、阳性率高,适宜在临床上推广使用。

Objective：To investigate the application value of IMS-smear anti-acid staining method and IMS-PCR technique. Methods： The anti-H37Rv immunoserums were immobilized on the surface of the active magnetic beads, which produced immunomagnetic beads. The immunomagnetic beads were used in capturing mycobacterium tuberculosis from sputum. Mycobacterium tuberculosis was eluted from immunomagnetic beads and smeared anti-acid stained and PCR. Results： IMS- smear staining method and IMS-PCR used in the detection of mycobacterium tuberculosis in sputum had higher positive rates than direct-smear and PCR. Conclusion： IMS-smear staining method and IMS-PCR technique are worthy to be recommended in clinical use due to easy and more sensitive.