化橘红道地性的初步研究

Studies on Genuineness of Exocarpium Citri Grandis

【目的】探讨道地化橘红药材——毛橘红形成原因。【方法】采用高效液相、随机扩增多态性DNA分析、等离子发射光谱法分别分析不同产地化橘红的柚皮苷含量、遗传距离以及土壤中Al、K、Ca、Fe、Ti、B、Mg、Mn 8种元素丰度,并应用SPSS 11.5统计软件作相关性分析。【结果】产地土壤表土层Ca丰度对柚皮苷含量有显著影响,心土层Al、K元素丰度差值与化橘红遗传距离呈显著相关。【结论】毛橘红形成的原因可能与当地盛产的礞石有一定关系。

Objective To explore the genuineness of Exocarpium Citri Grandis （ECG） from Huazhou city of Guangdong province. Methods We used the method of high performance liquid chromatography to detect the naringin content in ECG from different producing areas of Huazhou city. Random amplified polymorphic DNA analysis was used for the examination of genetic distance, and plasma-atomic emission spectrometry for the detection of soil elemental abundance of 8 elements such as aluminium （A1） , kalium （K） , calcium （Ca） , ferrum （Fe） , titanium （Ti）, boron （ B ）, magnesium （ Mg ）, and manganese （ Ma ）. The correlation of the above three parameters was analyzed by statistical software SPSS 11.5. Results Ca abundance in the surface soil layer had an obvious effect on the content of naringin, and the difference of A1 and K abundance in subsoil layer was correlated with the genetic distance of ECG. Conclusion The genuineness of ECG is probably related with the abundance of phlopopitum in the soil of producing areas of Huazhou city.