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ERK1/2信号通路在OX-LDL诱导的血管平滑肌细胞TLR4 mRNA表达中的作用 被引量：1

Role of ERK1/2 pathway in the oxidized low density lipoprotein induced TLR4 mRNA expression in vascular smooth muscle cells
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摘要 目的探讨ERK1/2信号通路在氧化性低密度脂蛋白(OX-LDL)诱导的血管平滑肌细胞(VSMCs)Toll样受体-4(TLR4)mRNA表达中的作用。方法采用贴块法培养大鼠VSMCs,在氧化低密度脂蛋白(OX-LDL)及PD98059(ERK1/2特异性抑制剂)作用下采用RT-PCR检测VSMCs TLR4 mRNA的表达,用Westernblotting检测ERK1/2磷酸化水平的变化。结果OX-LDL使VSMCs ERK1/2磷酸化水平升高;PD98059抑制ERK1/2的磷酸化;OX-LDL刺激VSMCs上调TLR4 mRNA的表达(P<0.05);PD98059预孵育后TLR4 mRNA的表达较单独OX-LDL刺激情况下降低(P<0.05)。结论OX-LDL通过或部分通过ERK1/2信号通路介导VSMCs TLR4 mRNA的表达。 Objective To investigate the role of ERK1/2 pathway in the Toll-like receptor 4（TLR4） mRNA expression in vascular smooth muscle cells（VSMCs） induced by oxidized low density lipoprotein（OX-LDL）.Methods VSMCs were isolated from the thoracic aorta of rats by explant technique.TLR4 mRNA expression in VSMCs stimulated with OX-LDL and PD98059（ERK1/2 specific inhibitor） were detected by RT-PCR.The variations of phosphorylation levels of ERK1/2 were detected by Western blotting.Results OX-LDL up-regulated the phosphorylation of ERK1/2;PD98059 down-regulated the phosphorylation of ERK1/2;the expression of TLR4 mRNA was up-regulated by OX-LDL stimulation in VSMCs（P0.05）,while it was diminished by PD98059 preincubation as compared to OX-LDL stimulation alone（P0.05）.Conclusion The expression of TLR4 mRNA in VSMCs could be mediated by OX-LDL at least in part via ERK1/2 pathways.
作者 徐斌 张延斌 许旭光 王大杰 潘德峰 李东野
机构地区 徐州医学院附属医院心内科
出处 《徐州医学院学报》 CAS 2010年第2期86-88,共3页 Acta Academiae Medicinae Xuzhou
关键词 动脉粥样硬化 血管平滑肌细胞 氧化性低密度脂蛋白 TOLL样受体-4 ERK1/2 atherosclerosis smooth muscle cells oxidized low density lipoprotein Toll-like receptor 4 ERK1/2
分类号 R543.3 [医药卫生—心血管疾病]
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参考文献11

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同被引文献12

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徐州医学院学报
2010年 第2期

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