草石蚕块茎总RNA提取及质量分析

Extraction and Quality Analysis of Total RNA in the Tuber of Stochys sieboldii Miq

采用3种方法提取草石蚕块茎的总RNA,以期筛选出适合草石蚕块茎总RNA提取的最佳方法。结果表明,RNAiso Reagent法提取草石蚕块茎总RNA的28S和18S条带清晰明亮,RNA无降解,无DNA污染,质量较好。王艳红法和TRIzolR Reagent一步提取法提取总RNA的28S和18S条带较暗,提取的总RNA有降解现象。根据紫外分光光度计检测结果分析,3种方法提取草石蚕块茎总RNA的纯度都较好。从提取RNA的产量来看,RNAiso Reagent法提取总RNA的浓度最高,为1112μg/mL,王艳红法次之,TRIzolR Reagent一步提取法最低。因此,RNAiso Reagent法是草石蚕块茎总RNA提取的最佳方法。

Three methods were compared for the extraction of total RNA from the tuber of Stochys sieboldii Miq in this paper. The results showed that the 28 S and 18 S bands were strong, ＇and the quality of the total RNA was high without degradation and DNA contamination, when the total RNA extracted by method 1 was detected with non-denaturing gel electrophoresis. While the 28 S and 18 S bands extracted by method 2 and method 3 were dark, and their total RNA degraded. The purity of total RNA extracted by the three methods was high according to the results of UV spectrophotometer detection. The concentration of the total RNA by method 1 was the highest （1 112 μg/mL）, next came method 2, and then method 3. Therefore, method 1 was optimal for total RNA extraction of the tuber of Stochys sieboldii Miq.