摘要
为了探讨氰戊菊酯对鲫鱼的影响,用不同浓度的氰戊菊酯(5、10、20、40μg/L)分别处理鲫鱼4、8、12、16d后,对肝胰脏、鳃中的超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、谷胱甘肽硫转移酶(GSTs)活性的变化进行了检测。结果表明,低浓度的氰戊菊酯能激活鲫鱼肝胰脏和鳃中的超氧化物歧化酶(SOD)活性,但随着氰戊菊酯浓度的增加和时间的延长,SOD酶活性受到不同程度的抑制;肝胰脏和鳃中AKP和ACP酶活性均随氰戊菊酯浓度的增加和染毒时间的延长而降低。其中在染毒后4d,肝胰脏AKP酶活性随着氯戊菊酯浓度升高而显著或极显著降低。肝胰脏ACP酶活性在染毒后12d和16d,试验组与对照组差异显著。鳃组织ACP活性试验组与对照组相比,大部分没有显著差异。氰戊菊酯对鲫鱼肝胰脏GSTs活性产生较强的影响,存在剂量-效应和时间-效应关系。鲫鱼肝胰脏和鳃中的SOD、AKP,肝胰脏中ACP、GSTs活性均可以用作其组织细胞受农药胁迫的生物标志物,鲫鱼可作为一种淡水水体污染的监测生物。
The effects of different concentration of fenvalerate (5,10,20,40μg/L)on activities of superoxide dismutase (SOD),alkaline phosphatase (AKP), acid phosphatase (ACP) and glntathione S-transferases (GSTs) were investigated in liver and gill of crucian(Carassius auratus) for 4, 8,12 and 16 days after exposure. The results showed that the activities of SOD increased in low fenvalerate levels. Then the activities of SOD decreased as the increase in fenvalerate concentration with the time extension. The AKP and ACP activities in liver and gill decreased as the increase in fenvalerate concentration with the time extension. After 4d, the activities of AKP in liver decreased obviously with the concentration enhancing (P〈0.05). After 12 and 16d, the activities of ACP in liver significantly reduced(P〈0.05). While ACP activities in gill showed no significantly difference (P〉0.05). Meanwhile, GSTs activities in liver and gill changed obviously with the fenvalerate contamination. The time-response and dose-response relationship existed between fenvalerate and activities of GSTs in liver and gill. The results suggested that fenvalerate could induce the activities of enzymes related with immune. Under the laboratory simulating condi- tions, the inchoate changes in SOD,AKP,ACP,GSTs activity in liver and gill could be regarded as sensitive indices of fenvalerate to the immune toxicity to the crucian (Carassius auratus) and Carasslus auratu could serve as an indicating organism of fresh water pollution environment.
出处
《湖北农业科学》
北大核心
2010年第2期412-415,442,共5页
Hubei Agricultural Sciences
基金
苏州科技学院科研基金(E1103)