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虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆 被引量：1

Construction of cDNA Library of Hoplobatrachus rugulosus Skin Tissue and Clone of the cDNA Encoding Antimicrobial Peptides tigerinin-HRs
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摘要 运用SMART技术成功构建了虎纹蛙(Hoplobatrachus rugulosus)皮肤组织全长cDNA文库。根据蛙科动物抗菌肽的信号肽保守序列设计简并引物,以PCR法成功从文库中随机挑选的阳性克隆中筛选出2个抗菌肽cDNA序列,命名为tigerinin-HR1和tigerinin-HR2。这2个抗菌肽cDNA序列是首次从虎纹蛙皮肤组织中克隆出的抗菌肽编码基因。 A full length cDNA library of Hoplobatrachus rugulosus skin tissue was constructed with the SMART technique.A special primer was designed from a highly conserved domain of the signal peptide of antimicrobial peptide encoding cDNAs from ranid frogs.Two different cDNA sequences encoding precursor proteins named tigerinin-HR1 and tigerinin-HR2 were screened from the library by PCR.tigerinin-HR1 and tigerinin-HR2 were the first found encoding cDNA sequences of antimicrobial peptide from H.rugulosus.
作者 王辉 张秀清 胡玉红 孙君社
机构地区 中国农业大学食品科学与营养工程学院 河北师范大学生命科学学院 河北师范大学实验中心
出处 《华北农学报》 CSCD 北大核心 2010年第1期61-67,共7页 Acta Agriculturae Boreali-Sinica
关键词 虎纹蛙 CDNA文库 抗菌肽 tigerinin Hoplobatrachus rugulosus cDNA library Antimicrobial peptides tigerinin
分类号 Q785 [生物学—分子生物学]
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参考文献25

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二级参考文献12

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华北农学报
2010年 第1期

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