摘要
根据GenBank中公布的小反刍兽疫病毒的H基因或全基因序列,利用软件Primer Premier5.0设计引物,扩增片段长度为477bp,建立区分小反刍兽疫疫苗毒与基因4系野毒的RT-PCR检测方法,并用于临床检测。结果表明,建立的RT-PCR鉴别诊断方法能特异性区分疫苗株Nigeria75/1与基因4系PPRV,与基因3系、牛瘟病毒、犬瘟热病毒等同属病毒无交叉反应;最低可以检测到浓度为7.7×10-5ng/μL的RNA模板;该方法操作简单,耗时短,可以初步用于临床鉴别诊断。
Based on the H gene sequence of peste des petits ruminants virus（PPRV）published in GenBank,a pair of special primers were designed by Primer Premier 5.0,the fragment of amplification was 477 bp in length.The establishment of RT-PCR assay aimed to differentiate vaccine and field isolates of 4-type peste des petits ruminants virus.By using the one-step RT-PCR,the wild-type and vaccine strains of PPRV in clinical samples were detected and accurately distinguished.The detection limit concentration of RNA was 7.7×10-5 ng/μL.
出处
《动物医学进展》
CSCD
北大核心
2010年第3期17-20,共4页
Progress In Veterinary Medicine
基金
农业部948项目(2006-G57(3))
关键词
RT-PCR
鉴别
小反刍兽疫病毒
RT-PCR
differentiation
Peste des petits ruminants virus（PPRV）
